Evidence map›Paper›PMID 34095865›Full record

ArticleSTAR protocols2021

Volume measurement and biophysical characterization of mounds in epithelial monolayers after intracellular bacterial infection.

Effie E Bastounis, Prathima Radhakrishnan, Christopher K Prinz, Julie A Theriot

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Effie E BastounisDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
Prathima RadhakrishnanDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
Christopher K PrinzDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
Julie A TheriotDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Funding

Actin-Based Motility of a Bacterial PathogenR37AI036929 · NIAID · UNIVERSITY OF WASHINGTON · PI THERIOT, JULIE A. · 2010 to 2019
$3.0M
NIAID NIH HHS R37 AI036929
6 · The paper itself

Abstract

Mechanical forces are important in (patho)physiological processes, including how host epithelial cells interact with intracellular bacterial pathogens. As these pathogens disseminate within host epithelial monolayers, large mounds of infected cells are formed due to the forceful action of surrounding uninfected cells, limiting bacterial spread across the basal cell monolayer. Here, we present a protocol for mound volume measurement and biophysical characterization of mound formation. Modifications to this protocol may be necessary for studying different host cell types or pathogenic organisms. For complete details on the use and execution of this protocol, please refer to Bastounis et al. (2021).

Indexed as

AnimalsBacteriaBacterial InfectionsBacteriological TechniquesBiophysical PhenomenaCell Culture TechniquesCell LineCells, CulturedDogsEpithelial CellsHost-Pathogen InteractionsHumansMadin Darby Canine Kidney CellsBiophysicsCell BiologyCell cultureImmunologyMicrobiologyMicroscopy

Identifiers

PMID34095865
PMCPMC8165451

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.