ArticleFrontiers in cell and developmental biology2021
Integrating Spatial Transcriptomics and Single-Cell RNA-seq Reveals the Gene Expression Profling of the Human Embryonic Liver.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 38 citations in OpenAlex.
- Autosomal Dominant Erythrocytosis Caused by Non-Renal Erythropoietin (EPO) Due to EPO c.-136 G>A Germline Mutation.American journal of hematology · 2026Article
- Comprehensive circRNA expression profile and hub genes screening during human liver development.Annals of medicine · 2025Article
- PoweREST: Statistical power estimation for spatial transcriptomics experiments to detect differentially expressed genes between two conditions.PLoS computational biology · 2025Article
- Spatial genomics: mapping human steatotic liver disease.Nature reviews. Gastroenterology & hepatology · 2024Review
- Article
- Single-cell Technologies Provide Novel Insights into Liver Physiology and Pathology.Journal of clinical and translational hepatology · 2024Review
- Single-nucleus transcriptome sequencing reveals hepatic cell atlas in pigs.BMC genomics · 2023Article
- Spatial transcriptomics in development and disease.Molecular biomedicine · 2023Review
- Spatial Single-Cell Technologies for Exploring Gastrointestinal Tissue Transcriptome.Comprehensive Physiology · 2023Article
- Advances in spatial transcriptomics and related data analysis strategies.Journal of translational medicine · 2023Review
- Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.Scientific reports · 2023Article
- Review
- SPASCER: spatial transcriptomics annotation at single-cell resolution.Nucleic acids research · 2023Article
- Spatially resolved transcriptomics: advances and applications.Blood science (Baltimore, Md.) · 2023Review
- Roles of Skeletal Muscle in Development: A Bioinformatics and Systems Biology Overview.Advances in anatomy, embryology, and cell biology · 2023Article
- Empirical single-cell tracking and cell-fate simulation reveal dual roles of p53 in tumor suppression.eLife · 2022Article
- Narrative engineering of the liver.Current opinion in genetics & development · 2022Review
- Spatial transcriptomics: new dimension of understanding biological complexity.Biophysics reports · 2022Article
- Dynamic 3D genome reorganization during development and metabolic stress of the porcine liver.Cell discovery · 2022Article
- Hepatic fibrosis 2022: Unmet needs and a blueprint for the future.Hepatology (Baltimore, Md.) · 2022Review
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Authors and funding
10 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The liver is one of vital organs of the human body, and it plays an important role in the metabolism and detoxification. Moreover, fetal liver is one of the hematopoietic places during ontogeny. Understanding how this complex organ develops during embryogenesis will yield insights into how functional liver replacement tissue can be engineered and how liver regeneration can be promoted. Here, we combine the advantages of single-cell RNA sequencing and Spatial Transcriptomics (ST) technology for unbiased analysis of fetal livers over developmental time from 8 post-conception weeks (PCW) and 17 PCW in humans. We systematically identified nine cell types, and defined the developmental pathways of the major cell types. The results showed that human fetal livers experienced blood rapid growth and immigration during the period studied in our experiments, and identified the differentially expressed genes, and metabolic changes in the developmental process of erythroid cells. In addition, we focus on the expression of liver disease related genes, and found that 17 genes published and linked to liver disease mainly expressed in megakaryocyte and endothelial, hardly expressed in any other cell types. Together, our findings provide a comprehensive and clear understanding of the differentiation processes of all main cell types in the human fetal livers, which may provide reference data and information for liver disease treatment and liver regeneration.
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