Evidence map›Paper›PMID 34094461›Full record

ArticleChemical science2020

A click-flipped enzyme substrate boosts the performance of the diagnostic screening for Hunter syndrome.

Markus Schwarz, Philipp Skrinjar, Michael J Fink, Stefan Kronister, Thomas Mechtler, Panagiotis I Koukos, Alexandre M J J Bonvin, David C Kasper, Hannes Mikula

Open access · diamondAbstract read
In one paragraph

Article in Chemical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Markus SchwarzInstitute of Applied Synthetic Chemistry, TU Wien Getreidemarkt 9 1060 Vienna Austria hannes.mikula@tuwien.ac.at.
Philipp SkrinjarInstitute of Applied Synthetic Chemistry, TU Wien Getreidemarkt 9 1060 Vienna Austria hannes.mikula@tuwien.ac.at.
Michael J FinkDepartment of Chemistry and Chemical Biology, Harvard University 12 Oxford Street Cambridge MA 02138 USA.ORCID https://orcid.org/0000-0003-0023-8767
Stefan KronisterInstitute of Applied Synthetic Chemistry, TU Wien Getreidemarkt 9 1060 Vienna Austria hannes.mikula@tuwien.ac.at.ORCID https://orcid.org/0000-0002-7076-6904
Thomas MechtlerARCHIMED Life Science GmbH Leberstraße 20 1110 Vienna Austria.
Panagiotis I KoukosBijvoet Centre for Biomolecular Research, Faculty of Science - Chemistry, Utrecht University Padualaan 8 3584CH Utrecht The Netherlands.
Alexandre M J J BonvinBijvoet Centre for Biomolecular Research, Faculty of Science - Chemistry, Utrecht University Padualaan 8 3584CH Utrecht The Netherlands.
David C KasperARCHIMED Life Science GmbH Leberstraße 20 1110 Vienna Austria.
Hannes MikulaInstitute of Applied Synthetic Chemistry, TU Wien Getreidemarkt 9 1060 Vienna Austria hannes.mikula@tuwien.ac.at.ORCID https://orcid.org/0000-0002-9218-9722
TU Wien · ATUtrecht University · NLHarvard University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report on the unexpected finding that click modification of iduronyl azides results in a conformational flip of the pyranose ring, which led to the development of a new strategy for the design of superior enzyme substrates for the diagnostic assaying of iduronate-2-sulfatase (I2S), a lysosomal enzyme related to Hunter syndrome. Synthetic substrates are essential in testing newborns for metabolic disorders to enable early initiation of therapy. Our click-flipped iduronyl triazole showed a remarkably better performance with I2S than commonly used

Identifiers

PMID34094461
PMCPMC8163285
OpenAlexW3094333187

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.