ReviewGreen chemistry : an international journal and green chemistry resource : GC2021
Applied biocatalysis beyond just buffers - from aqueous to unconventional media. Options and guidelines.
Review in Green chemistry : an international journal and green chemistry resource : GC, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed.
- An Aryl Alcohol Oxidase-Peroxygenase Catalyst System Enables Efficient Alcohol to Aldehyde Transformation.Organic process research & development · 2026Article
- Toward Sustainability: Intensification of Light-Driven Whole Cell Biocatalysis.Chembiochem : a European journal of chemical biology · 2026Review
- Biocatalysis Under Reduced Pressure; Two-Step, One-Pot Amide Synthesis Using an Immobilised Transaminase/Lipase Cascade in Combination With By-Product Removal.Chembiochem : a European journal of chemical biology · 2026Article
- Biocatalytic reduction of nitro-sulfonamides: enzyme engineering, process integration, and sustainable pathways toFrontiers in bioengineering and biotechnology · 2026Review
- Molar-Scale Phenolic Acid Decarboxylation Using Thermostable Biocatalysts and Enzyme-Compatible Deep Eutectic Solvents.ChemSusChem · 2025Article
- Reaction Engineering of In Vitro Natural Product Biosynthesis: Challenges and Strategies.Chembiochem : a European journal of chemical biology · 2025Review
- Comparative Roles of Hydrogels, Deep Eutectic Solvents, and Ionic Liquids in Enzyme-Based Biosensors, Bioelectronics and Biomimetics Devices.ACS measurement science au · 2025Review
- Review
- Optimization of lipase activity in Aspergillus niger C2J6 whole cells using choline chloride ethylene glycol deep eutectic solvent.Scientific reports · 2025Article
- Article
- Redox Biocatalysis in Lidocaine-Based Hydrophobic Deep Eutectic Solvents: Non-Conventional Media Outperform Aqueous Conditions.ChemSusChem · 2025Article
- Deep eutectic solvents assisted laccase pretreatment for improving enzymatic hydrolysis of corn stover.Bioprocess and biosystems engineering · 2025Article
- Concatenating Microbial, Enzymatic, and Organometallic Catalysis for Integrated Conversion of Renewable Carbon Sources.JACS Au · 2024Review
- Overcoming a Conceptual Limitation of Industrial ε-Caprolactone Production via Chemoenzymatic Synthesis in Organic Medium.ChemSusChem · 2024Article
- Mechanochemistry for Organic and Inorganic Synthesis.ACS organic & inorganic Au · 2024Review
- Efficient Synthesis of Chiral Aryl Alcohol with a Novel Kosakonia radicincitans Isolate in Tween 20/L-carnitine: Lysine-Containing Synergistic Reaction System.Applied biochemistry and biotechnology · 2024Article
- Harnessing the potential of deep eutectic solvents in biocatalysis: design strategies using COFrontiers in chemistry · 2024Article
- Interdisciplinary development of an overall process concept from glucose to 4,5-dimethyl-1,3-dioxolane via 2,3-butanediol.Communications chemistry · 2023Article
- Solvent-free enzymatic synthesis and evaluation of vanillyl propionate as an effective and biocompatible preservative.Bioprocess and biosystems engineering · 2023Article
- Recent Advances in Overexpression of Functional Recombinant Lipases.Molecular biotechnology · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In nature, enzymes conventionally operate under aqueous conditions. Because of this, aqueous buffers are often the choice for reaction media when enzymes are applied in chemical synthesis. However, to meet the demands of an industrial application, due to the poor water solubility of many industrially relevant compounds, an aqueous reaction system will often not be able to provide sufficient substrate loadings. A switch to a non-aqueous solvent system can provide a solution, which is already common for lipases, but more challenging for biocatalysts from other enzyme classes. The choices in solvent types and systems, however, can be overwhelming. Furthermore, some engineering of the protein structure of biocatalyst formulation is required. In this review, a guide for those working with biocatalysts, who look for a way to increase their reaction productivity, is presented. Examples reported clearly show that bulk water is not necessarily required for biocatalytic reactions and that clever solvent systems design can support increased product concentrations thereby decreasing waste formation. Additionally, under these conditions, enzymes can also be combined in cascades with other, water-sensitive, chemical catalysts. Finally, we show that the application of non-aqueous solvents in biocatalysis can actually lead to more sustainable processes. At the hand of flowcharts, following simple questions, one can quickly find what solvent systems are viable.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.