ArticleNature protocols2021
Whole-brain functional ultrasound imaging in awake head-fixed mice.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
58 citing papers in PubMed.
- Trimodal ultrasound imaging of brain-tumor interaction.iScience · 2026Article
- Longitudinal Awake Mouse Brain Imaging Using Functional Ultrasound and Functional Ultrasound Localization Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Microbubble Track-Based Functional Ultrasound Localization Microscopy in Awake Mice.IEEE transactions on medical imaging · 2026Article
- Contrast-Free Ultrasound Microvascular Imaging via Radiality and Similarity Weighting.IEEE transactions on ultrasonics · 2026Article
- Functional Ultrasound Localization Microscopy on Freely Moving Rats.Research square · 2026Article
- A hemispheric decoding principle for vestibular heading perception in the posterior sylvian area.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Ultrasound Modulation of Visual Circuits in Mice Independent of Auditory Confound.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Reduced mediodorsal thalamus activity underlies aberrant belief dynamics in a genetic mouse model of schizophrenia.Nature neuroscience · 2026Article
- Imaging of Neurovascular Activity during Motor Responses Evoked by Displacement-guided Focused Ultrasound (DgFUS) Neuromodulation in Mice.Research square · 2026Article
- Hemodynamic and Electrophysiological Progression of the Rose Bengal Photothrombotic Stroke Model in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Mapping Microvascular Flow via Radon Transform Ultrasound: Technical Advances and Pilot Application.BME frontiers · 2026Article
- Article
- Microfluidics-Enabled Simultaneous Imaging of Neural Activity and Behavior in Chemically Stimulated, Head-FixedbioRxiv : the preprint server for biology · 2025Article
- Robust single-trial decoding of physical self-motion from hemodynamic signals in the brain measured by functional ultrasound imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Improving Imaging Field of View of 3-D Transcranial Rat Brain Super-Resolution With Robotic Registered Compounding and Nonrigid Deformation Correction.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2025Article
- Ultrasound Super-Resolution Imaging of Neonatal Cerebral Vascular Reorganization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Physio-fUS: a tissue-motion based method for heart and breathing rate assessment in neurofunctional ultrasound imaging.EBioMedicine · 2025Article
- Large Scale in vivo Acquisition, Segmentation and 3D Reconstruction of Cortical Vasculature usingNeuroinformatics · 2025Article
- Functional Ultrasound Imaging of Auditory Responses in Comatose Patients.Research (Washington, D.C.) · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Most brain functions engage a network of distributed regions. Full investigation of these functions thus requires assessment of whole brains; however, whole-brain functional imaging of behaving animals remains challenging. This protocol describes how to follow brain-wide activity in awake head-fixed mice using functional ultrasound imaging, a method that tracks cerebral blood volume dynamics. We describe how to set up a functional ultrasound imaging system with a provided acquisition software (miniScan), establish a chronic cranial window (timing surgery: ~3-4 h) and image brain-wide activity associated with a stimulus at high resolution (100 × 110 × 300 µm and 10 Hz per brain slice, which takes ~45 min per imaging session). We include codes that enable data to be registered to a reference atlas, production of 3D activity maps, extraction of the activity traces of ~250 brain regions and, finally, combination of data from multiple sessions (timing analysis averages ~2 h). This protocol enables neuroscientists to observe global brain processes in mice.
Indexed as
Identifiers
34089019What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.