Evidence map›Paper›PMID 34088337›Full record

ArticleJournal of experimental & clinical cancer research : CR2021

LINC00511 drives invasive behavior in hepatocellular carcinoma by regulating exosome secretion and invadopodia formation.

Xueqiang Peng, Xinyu Li, Shuo Yang, Mingyao Huang, Shibo Wei, Yingbo Ma, Yan Li, Bo Wu, Hongyuan Jin, Bowen Li and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental & clinical cancer research : CR, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 52 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Xueqiang PengDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.ORCID https://orcid.org/0000-0003-3522-0348
Xinyu LiDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Shuo YangDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Mingyao HuangDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Shibo WeiDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Yingbo MaDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Yan LiDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Bo WuDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Hongyuan JinDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Bowen LiDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Shilei TangDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Qing FanDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Jingang LiuDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.
Liang YangDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China. 529687607@qq.com.
Hangyu LiDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China. sj_li_hangyu@sina.com.ORCID https://orcid.org/0000-0002-2570-1642
Fourth Affiliated Hospital of China Medical University · CN

Funding

National Natural Science Foundation of China 81472302National Natural Science Foundation of China 81871983National Natural Science Foundation of China 82003040Natural Science Foundation of Liaoning Province 2020-BS-103
6 · The paper itself

Abstract

backgroundTumor cells are known to release large numbers of exosomes containing active substances that participate in cancer progression. Abnormally expressed long noncoding RNAs (lncRNAs) have been confirmed to regulate multiple processes associated with tumor progression. However, the mechanism by which lncRNAs affect exosome secretion remains unclear.

methodsThe underlying mechanisms of long noncoding RNA LINC00511 (LINC00511) regulation of multivesicular body (MVB) trafficking, exosome secretion, invadopodia formation, and tumor invasion were determined through gene set enrichment analysis (GSEA), immunoblotting, nanoparticle tracking analysis, confocal colocalization analysis, electron microscopy, and invasion experiments.

resultsWe revealed that the tumorigenesis process is associated with a significant increase in vesicle secretion in hepatocellular carcinoma (HCC). Additionally, LINC00511 was significantly more highly expressed in HCC tissues and is related to vesicle trafficking and MVB distribution. We also found that in addition to the formation of invadopodia in HCC progression, abnormal LINC00511 induces invadopodia formation in HCC cells by regulating the colocalization of vesicle associated membrane protein 7 (VAMP7) and synaptosome associated protein 23 (SNAP23) to induce the invadopodia formation, which are key secretion sites for MVBs and control exosome secretion. Finally, we revealed that LINC0051-induced invadopodia and exosome secretion were involved in tumor progression.

conclusionsOur experiments revealed novel findings on the relationship between LINC00511 dysregulation in HCC and invadopodia production and exosome secretion. This is a novel mechanism by which LINC00511 regulates invadopodia biogenesis and exosome secretion to further promote cancer progression.

Indexed as

CarcinogenesisCarcinoma, HepatocellularDisease ProgressionExosomesGene Expression Regulation, NeoplasticHumansLiver NeoplasmsNeoplasm InvasivenessPodosomesQb-SNARE ProteinsQc-SNARE ProteinsRNA, Long NoncodingR-SNARE ProteinsQb-SNARE ProteinsQc-SNARE ProteinsRNA, Long NoncodingR-SNARE ProteinsSNAP23 protein, humanVAMP7 protein, humanExosomeHepatocellular carcinomaInvadopodiaLncRNAsMultivesicular body

Identifiers

PMID34088337
PMCPMC8176717
OpenAlexW3165774058

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.