Evidence map›Paper›PMID 34088325›Full record

ArticleReproductive biology and endocrinology : RB&E2021

The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro.

Qigui Xie, Yaxin Xing, Jianhong Zhou, Ling Wang, Jie Wu, Ri-Cheng Chian

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Qigui Xie *Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, Nanjing Medical University, Shanghai, China.
Yaxin Xing *Center for Reproductive Medicine, Shanghai Tenth People's Hospital of Tongji University, 301 Yanchang zhonglu, 200072, Shanghai, China.
Jianhong ZhouDepartment of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, Nanjing Medical University, Shanghai, China.
Ling WangCenter for Reproductive Medicine, Shanghai Tenth People's Hospital of Tongji University, 301 Yanchang zhonglu, 200072, Shanghai, China.
Jie WuDepartment of Obstetrics and Gynecology, Jiangsu Province Hospital, The First Affiliated Hospital with Nanjing Medical University, Nanjing Medical University, 300 Guangzhou Road, Nanjing, China. wujiemd@126.com.
Ri-Cheng ChianCenter for Reproductive Medicine, Shanghai Tenth People's Hospital of Tongji University, 301 Yanchang zhonglu, 200072, Shanghai, China. rchian@126.com.
Nanjing Medical University · CNShanghai Tenth People's Hospital · CNJiangsu Province Hospital · CNTongji University · CN

Funding

National Key R&D Program of China No. 2017YFC1002003The Ministry of Science and Technology of China No. 2017YFC1001601
6 · The paper itself

Abstract

backgroundLysophosphatidic acid-supplemented culture medium significantly increases the oocyte maturation rate in vitro. However, potential targets and pathways involved remain unknown.

methodsA total of 43 women, who underwent cesarean section and aged between 18 and 35 years with good health, were included in this study. Immature oocytes were obtained and cultured with 10 µM lysophosphatidic acid. After culture, oocyte maturation was assessed and oocytes and cumulus cells were collected for RNA sequencing. Hierarchical indexing for spliced alignment of transcripts 2 method was used to align clean reads to the human genome. The featureCounts and edgeR package were used to calculate gene expression and analyze differences between groups respectively. ClusterProfiler program was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis.

resultsOocyte maturation rate increased significantly following 48 h culture with lysophosphatidic acid. In cumulus cells, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in TNF signaling and insulin secretion pathways and downregulated genes were enriched in TNF signaling and cell adhesion molecules. In oocytes, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in MAPK signaling, gap junction, and cell cycle pathways and downregulated genes were enriched in MAPK signaling, estrogen signaling, RAP1 signaling, and gap junction pathways.

conclusionsLysophosphatidic acid in culture medium enhances human oocyte maturation in vitro and the identified some potential pathways may associate with oocyte maturation.

Indexed as

Culture MediaAdultCell CycleCumulus CellsDown-RegulationEpidermal Growth FactorEstrogensFemaleFollicle Stimulating HormoneGap JunctionsGene ExpressionGene OntologyHumansIn Vitro Oocyte Maturation TechniquesLuteinizing HormoneLysophospholipidsCulture MediaEpidermal Growth FactorEstrogensFollicle Stimulating HormoneLuteinizing Hormonelysophosphatidic acidLysophospholipidsMitogen-Activated Protein KinasesCumulus cellsFertilityIn vitro maturationLysophosphatidic acidOocytes

Identifiers

PMID34088325
PMCPMC8176579
OpenAlexW3132642897

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.