ArticleBiophysical journal2021
Myofiber stretch induces tensile and shear deformation of muscle stem cells in their native niche.
Article in Biophysical journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Time-of-day, satellite cells, and velocity collectively influence ex vivo isovelocity force production in mouse extensor digitorum longus muscle.Physiological reports · 2026Article
- Mechanobiological landscape of muscle stem cells.Skeletal muscle · 2026Review
- Compression bioreactor for cultured meat.Current research in food science · 2026Article
- Tenascin-C from the tissue microenvironment promotes muscle stem cell maintenance and function through Annexin A2.Communications biology · 2025Article
- In vivo mechano-tissue engineering by hydrogels capable of transmitting intercellular mechanical stress.Nature communications · 2025Article
- Article
- Muscle Stem Cell Microenvironment and Functions in Muscle Regeneration.Biomolecules · 2025Review
- Skeletal Muscle Tissue Engineering: From Tissue Regeneration to Biorobotics.Cyborg and bionic systems (Washington, D.C.) · 2025Review
- A review and empirical findings of fasciae and muscle interactions in low back pain.Frontiers in physiology · 2025Article
- Three-dimensional imaging studies in mice identify cellular dynamics of skeletal muscle regeneration.Developmental cell · 2024Article
- A Pharmacological Investigation of the TMEM16A Currents in Murine Skeletal Myogenic Precursor Cells.International journal of molecular sciences · 2024Article
- The important role of cellular mechanical microenvironment in engineering structured cultivated meat: Recent advances.Current research in food science · 2024Review
- Migration and differentiation of muscle stem cells are coupled by RhoA signalling during regeneration.Open biology · 2023Article
- Resistance exercise: a mighty tool that adapts, destroys, rebuilds and modulates the molecular and structural environment of skeletal muscle.Physical activity and nutrition · 2023Article
- An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.Cell stem cell · 2022Article
- Reduced growth rate of aged muscle stem cells is associated with impaired mechanosensitivity.Aging · 2022Article
- Fusion and beyond: Satellite cell contributions to loading-induced skeletal muscle adaptation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Review
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
Abstract
Muscle stem cells (MuSCs) are requisite for skeletal muscle regeneration and homeostasis. Proper functioning of MuSCs, including activation, proliferation, and fate decision, is determined by an orchestrated series of events and communication between MuSCs and their niche. A multitude of biochemical stimuli are known to regulate MuSC fate and function. However, in addition to biochemical factors, it is conceivable that MuSCs are subjected to mechanical forces during muscle stretch-shortening cycles because of myofascial connections between MuSCs and myofibers. MuSCs respond to mechanical forces in vitro, but it remains to be proven whether physical forces are also exerted on MuSCs in their native niche and whether they contribute to the functioning and fate of MuSCs. MuSC deformation in their native niche resulting from mechanical loading of ex vivo myofiber bundles was visualized utilizing mT/mG double-fluorescent Cre-reporter mouse and multiphoton microscopy. MuSCs were subjected to 1 h pulsating fluid shear stress (PFSS) with a peak shear stress rate of 6.5 Pa/s. After PFSS treatment, nitric oxide, messenger RNA (mRNA) expression levels of genes involved in regulation of MuSC proliferation and differentiation, ERK 1/2, p38, and AKT activation were determined. Ex vivo stretching of extensor digitorum longus and soleus myofiber bundles caused compression as well as tensile and shear deformation of MuSCs in their niche. MuSCs responded to PFSS in vitro with increased nitric oxide production and an upward trend in iNOS mRNA levels. PFSS enhanced gene expression of c-Fos, Cdk4, and IL-6, whereas expression of Wnt1, MyoD, Myog, Wnt5a, COX2, Rspo1, Vangl2, Wnt10b, and MGF remained unchanged. ERK 1/2 and p38 MAPK signaling were also upregulated after PFSS treatment. We conclude that MuSCs in their native niche are subjected to force-induced deformations due to myofiber stretch-shortening. Moreover, MuSCs are mechanoresponsive, as evidenced by PFSS-mediated expression of factors by MuSCs known to promote proliferation.
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