ArticlePloS one2021
Characterization of Brassica rapa metallothionein and phytochelatin synthase genes potentially involved in heavy metal detoxification.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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12 citing papers in PubMed, 29 citations in OpenAlex.
- Natural Defenses Against Cadmium Toxicity: Mechanisms and Emerging Strategies - A Review.Biological trace element research · 2026Review
- Drought Stress Response of Doubled Haploid Interspecific Rapeseed Hybrids at Germination and Flowering Stages.Biology · 2026Article
- Regulation of aluminum induced oxidative stress in Brassica via roles of sulfur metabolites and antioxidant responses mediated by a melatonin selenium nanocomposite.BMC plant biology · 2025Article
- Arbuscular Mycorrhizal Fungi Enhance Antioxidant Defense Systems in Sugarcane Under Soil Cadmium Stress.Plants (Basel, Switzerland) · 2025Article
- Molecular Mechanisms of Cadmium Stress Resistance in Vegetable Crops.International journal of molecular sciences · 2025Review
- Overexpression of NTPCS1 Enhances Zn Tolerance in Tobacco.Plants (Basel, Switzerland) · 2025Article
- The Contribution ofFoods (Basel, Switzerland) · 2024Article
- Characterization of aFrontiers in plant science · 2024Article
- Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants.International journal of molecular sciences · 2023Review
- A novel geneFrontiers in plant science · 2023Article
- Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress inInternational journal of molecular sciences · 2022Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brassica rapa is an important leafy vegetable that can potentially accumulate high concentrations of cadmium (Cd), posing a risk to human health. The aim of the present study was to identify cadmium detoxifying molecular mechanisms in B. rapa using a functional cloning strategy. A cDNA library constructed from roots of B. rapa plants treated with Cd was transformed into the Cd sensitive yeast mutant strain DTY167 that lacks the yeast cadmium factor (YCF1), and resistant yeast clones were selected on Cd containing media. Two hundred genes potentially conferring cadmium resistance were rescued from the surviving yeast clones and sequenced. Sequencing analysis revealed that genes encoding for metallothionein (MT)1, MT2a, MT2b and MT3, and phytochelatin synthase (PCS)1 and PCS2 accounted for 35.5%, 28.5%, 4%, 11.3%, 18.7% and 2%, respectively of the genes identified. MTs and PCSs expressing DTY167 cells showed resistance to Cd as well as to Zn. PCS1 expressing yeast cells were also more resistant to Pb compared to those expressing MTs or PCS2. RT-PCR results showed that Cd treatment strongly induced the expression levels of MTs in the root and shoot. Furthermore, the different MTs and PCSs exhibited tissue specific expression. The results indicate that MTs and PCS genes potentially play a central role in detoxifying Cd and other toxic metals in B. rapa.
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