ArticlePloS one2021
ACRBP (Sp32) is involved in priming sperm for the acrosome reaction and the binding of sperm to the zona pellucida in a porcine model.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Medium Composition Determines the Dynamics of Boar In Vitro Sperm Capacitation-Associated Events.International journal of molecular sciences · 2026Article
- Role of the CaCells · 2025Article
- Functional Methods for Studying Sperm-Zona Pellucida Interaction in Mammals.Methods and protocols · 2025Review
- Article
- Calcium Channels and Modulators as Potential Therapeutic Targets for Contraceptives and Male Fertility: A Scoping Review.Open access journal of contraception · 2025Review
- Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases.International journal of legal medicine · 2024Review
- Male infertility and perfluoroalkyl and poly-fluoroalkyl substances: evidence for alterations in phosphorylation of proteins and fertility-related functional attributes in bull spermatozoa†.Biology of reproduction · 2024Article
- Differential Proteomic Analysis of Human Sperm: A Systematic Review to Identify Candidate Targets to Monitor Sperm Quality.The world journal of men's health · 2024Article
- Characterization of acrosin and acrosin binding protein as novel CRISP2 interacting proteins in boar spermatozoa.Andrology · 2023Article
- Identification of protein candidates in spermatozoa of water buffalo (Frontiers in cell and developmental biology · 2023Article
- Immunohistochemistry Study of OY-TES-1 Location in Fetal and Adult Human Tissues.Journal of healthcare engineering · 2022Article
- piR-121380 Is Involved in Cryo-Capacitation and Regulates Post-Thawed Boar Sperm Quality Through Phosphorylation of ERK2Frontiers in cell and developmental biology · 2021Article
- Research Progress on the Microregulatory Mechanisms of Fertilization: A Review.In vivo (Athens, Greece)Review
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In boar sperm, we have previously shown that capacitation is associated with the appearance of the p32 tyrosine phosphoprotein complex. The principal tyrosine phosphoprotein involved in this complex is the acrosin-binding protein (ACRBP), which regulates the autoconversion of proacrosin to intermediate forms of acrosin in both boar and mouse sperm. However, the complete biological role of ACRBP has not yet been elucidated. In this study, we tested the hypothesis that tyrosine phophorylation and the presence of the ACRBP in the sperm head are largely necessary to induce capacitation, the acrosome reaction (AR) and sperm-zona pellucida (ZP) binding, all of which are necessary steps for fertilization. In vitro fertilization (IVF) was performed using matured porcine oocytes and pre-capacitated boar sperm cultured with anti-phosphotyrosine antibodies or antibodies against ACRBP. Anti-ACRBP antibodies reduced capacitation and spontaneous AR (P<0.05). Sperm-ZP binding declined in the presence of anti-phosphotyrosine or anti-ACRBP antibodies. The localisation of anti-ACRBP antibodies on the sperm head, reduced the ability of the sperm to undergo the AR in response to solubilized ZP or by inhibiting the sarco/endoplasmic reticulum Ca2+-ATPase. These results support our hypothesis that tyrosine phosphorylated proteins and ACRBP are present upon the sperm surface in order to participate in sperm-ZP binding, and that ACRBP upon the surface of the sperm head facilitates capacitation and the AR in the porcine.
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