ArticlePloS one2021
Circadian misalignment by environmental light/dark shifting causes circadian disruption in colon.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- Sleep restriction induces histoarchitectural and cellular remodeling in the rat distal colon: a peripheral consequence of circadian disruption.Biochemistry and biophysics reports · 2026Article
- Mechanistic insights into pancreatic cancer progression from circadian rhythm disruption and gut microbiota dysbiosis (Review).International journal of molecular medicine · 2026Review
- Sex differences in the effects of shift work-like schedules on gut microbiome and intestinal barrier in relation to stroke survival.PloS one · 2026Article
- Impact of peripheral circadian misalignment and alcohol on the resiliency of intestinal barrier and microbiota.Gut microbes · 2025Article
- Circadian Rhythms in Gastroenterology: The Biological Clock's Impact on Gut Health.Gastroenterology · 2025Review
- Total darkness activated intestinal clock system and improved intestinal barrier function in growing rabbits.BMC microbiology · 2025Article
- The circadian rhythm as therapeutic target in inflammatory bowel disease.Journal of the Canadian Association of Gastroenterology · 2025Article
- Article
- Time-restricted feeding leads to sex- and organ-specific responses in the murine digestive system.PloS one · 2025Article
- Disruption of the intestinal clock drives dysbiosis and impaired barrier function in colorectal cancer.Science advances · 2024Article
- Chronobiology of Cancers in the Liver and Gut.Cancers · 2024Review
- Effect of Alcohol on Clock Synchrony and Tissue Circadian Homeostasis in Mice.Molecular nutrition & food research · 2024Article
- Effect of Urolithin A on the Improvement of Circadian Rhythm Dysregulation in Intestinal Barrier Induced by Inflammation.Nutrients · 2024Article
- Morning light treatment for inflammatory bowel disease: a clinical trial.BMC gastroenterology · 2024Article
- Evidence that the loss of colonic anti-microbial peptides may promote dysbiotic Gram-negative inflammaging-associated bacteria in aging mice.Frontiers in aging · 2024Article
- Circadian disruption dysregulates lung gene expression associated with inflammatory lung injury.Frontiers in immunology · 2024Article
- Melatonin Prevents Alcohol- and Metabolic Dysfunction- Associated Steatotic Liver Disease by Mitigating Gut Dysbiosis, Intestinal Barrier Dysfunction, and Endotoxemia.Antioxidants (Basel, Switzerland) · 2023Review
- Abrupt Photoperiod Changes Differentially Modulate Hepatic Antioxidant Response in Healthy and Obese Rats: Effects of Grape Seed Proanthocyanidin Extract (GSPE).International journal of molecular sciences · 2023Article
- Circadian rhythms in colonic function.Frontiers in physiology · 2023Review
- Grape Seed Proanthocyanidins Mitigate the Disturbances Caused by an Abrupt Photoperiod Change in Healthy and Obese Rats.Nutrients · 2022Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPhysiological circadian rhythms (CRs) are complex processes with 24-hour oscillations that regulate diverse biological functions. Chronic weekly light/dark (LD) shifting (CR disruption; CRD) in mice results in colonic hyperpermeability. However, the mechanisms behind this phenomenon are incompletely understood. One potential innovative in vitro method to study colonic CRs are colon organoids. The goals of this study were to utilize circadian clock gene Per2 luciferase reporter (Per2::Luc) mice to measure the effects of chronic LD shifting on colonic tissue circadian rhythmicity ex vivo and to determine if organoids made from shifted mice colons recapitulate the in vivo phenotype.
methodsNon-shifted (NS) and shifted (S) BL6 Per2::Luc mice were compared after a 22-week experiment. NS mice had a standard 12h light/12h dark LD cycle throughout. S mice alternated 12h LD patterns weekly, with light from 6am-6pm one week followed by shifting light to 6pm-6am the next week for 22 weeks. Mice were tested for intestinal permeability while colon tissue and organoids were examined for CRs of bioluminescence and proteins of barrier function and cell fate.
resultsThere was no absolute difference in NS vs. S 24h circadian period or phase. However, chronic LD shifting caused Per2::Luc S mice colon tissue to exhibit significantly greater variability in both the period and phase of Per2::Luc rhythms than NS mice colon tissue and organoids. Chronic LD shifting also resulted in increased colonic permeability of the Per2::Luc mice as well as decreased protein markers of intestinal permeability in colonic tissue and organoids from shifted Per2:Luc mice.
conclusionsOur studies support a model in which chronic central circadian disruption by LD shifting alters the circadian phenotype of the colon tissue and results in colon leakiness and loss of colonic barrier function. These CRD-related changes are stably expressed in colon stem cell derived organoids from CRD mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.