Evidence map›Paper›PMID 34086280›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria.

Cordelia A Weiss, Wade C Winkler

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Cordelia A WeissDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA.
Wade C WinklerDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA. wwinkler@umd.edu.
University of Maryland, College Park · US

Funding

Post-initiation regulatory mechanisms controlling ethanolamine utilizationR01AI110432 · NIAID · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI GARSIN, DANIELLE A, WINKLER, WADE · 2015 to 2019
$2.2M
NIAID NIH HHS R01 AI110432
6 · The paper itself

Abstract

Riboswitches are a class of noncoding RNAs that regulate gene expression in response to changes in intracellular metabolite concentrations. When riboswitches are placed upstream of genetic reporters, the degree of reporter activity reflects the relative abundance of the metabolite that is sensed by the riboswitch. This method describes how reporters for live cell imaging, such as yellow fluorescent protein (YFP), can be placed under genetic control by metabolite-sensing riboswitches in the bacterium Bacillus subtilis. Specifically, a protocol for generating a fluorescent YFP reporter, based on a c-di-GMP responsive riboswitch, is outlined below.

Indexed as

Genes, Reporter5' Untranslated RegionsAptamers, NucleotideBacillus subtilisBacterial ProteinsBase SequenceCyclic GMPGene Expression Regulation, BacterialIntravital MicroscopyLigandsLuminescent ProteinsNucleic Acid ConformationPlasmidsRiboswitchTransformation, Bacterial5' Untranslated RegionsAptamers, NucleotideBacterial Proteinsbis(3',5')-cyclic diguanylic acidCyclic GMPLigandsLuminescent ProteinsRiboswitchyellow fluorescent protein, BacteriaBacillus subtilisCyclic di-GMPFluorescence microscopyLive cell imagingMetabolite biosensorRiboswitch

Identifiers

PMID34086280
OpenAlexW3164828752

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.