Evidence map›Paper›PMID 34086279›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging.

Qikun Yu, Ru Zheng, Manojkumar Narayanan, Mingxu You

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Qikun YuDepartment of Chemistry, University of Massachusetts, Amherst, MA, USA.
Ru ZhengDepartment of Chemistry, University of Massachusetts, Amherst, MA, USA.
Manojkumar NarayananDepartment of Chemistry, University of Massachusetts, Amherst, MA, USA.
Mingxu YouDepartment of Chemistry, University of Massachusetts, Amherst, MA, USA. mingxuyou@umass.edu.
University of Massachusetts Amherst · US

Funding

Genetically-encoded fluorescent RNA sensors for measuring transport of antibiotics into the cytoplasm of Gram-negative pathogens and development of efflux pump inhibitorsR01AI136789 · NIAID · HARVARD MEDICAL SCHOOL · PI LORY, STEPHEN · 2018 to 2022
$3.6M
NIAID NIH HHS R01 AI136789
6 · The paper itself

Abstract

Fluorescence-based tools are invaluable in studying cellular functions. Traditional small molecule or protein-based fluorescent sensors have been widely used for the cellular imaging, but the choice of targets is still limited. Recently, fluorogenic RNA-based sensors gained lots of attention. This novel sensor system can function as a general platform for various cellular targets. Here, we describe the steps to rationally design, optimize, and apply fluorogenic RNA-based sensors, using the intracellular imaging of tetracycline in living E. coli cells as an example.

Indexed as

Allosteric RegulationAptamers, NucleotideBenzyl CompoundsBiosensing TechniquesCloning, MolecularComputer SimulationDrug DesignEscherichia coliFluorescent DyesImidazolinesIntravital MicroscopyMolecular StructureNucleic Acid ConformationTetracycline3,5-difluoro-4-hydroxybenzylidene imidazolinoneAptamers, NucleotideBenzyl CompoundsFluorescent DyesImidazolinesTetracyclineAllosteric RNA sensorAntibioticsAptamersFluorogenic RNAGenetically encodedIntracellular imaging

Identifiers

PMID34086279
OpenAlexW3165551296

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.