ArticleApplied and environmental microbiology2021
A Quantitative Metagenomic Sequencing Approach for High-Throughput Gene Quantification and Demonstration with Antibiotic Resistance Genes.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Quantitative metagenomics using a portable protocol.Applied and environmental microbiology · 2026Article
- Bio-Based Fertilizers from Waste: Nutrient Recovery, Soil Health, and Circular Economy Impacts.Toxics · 2026Review
- Presence of antimicrobial resistance genes in biofilms from swine drinking water pipes before and after treatment with peracetic acid.Frontiers in microbiology · 2026Article
- Evaluating Quantitative Metagenomics for Environmental Monitoring of Antibiotic Resistance and Establishing Detection Limits.Environmental science & technology · 2025Article
- Development of a quantitative metagenomic approach to establish quantitative limits and its application to viruses.Nucleic acids research · 2025Article
- A novel synthetic nucleic acid mixture for quantification of microbes by mNGS.Microbial genomics · 2024Article
- Vibrio-Sequins - dPCR-traceable DNA standards for quantitative genomics of Vibrio spp.BMC genomics · 2023Article
- A Metagenomic Approach for Characterizing Antibiotic Resistance Genes in Specific Bacterial Populations: Demonstration with Escherichia coli in Cattle Manure.Applied and environmental microbiology · 2022Article
- A review of the emergence of antibiotic resistance in bioaerosols and its monitoring methods.Re/views in environmental science and bio/technology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive microbial risk assessment requires high-throughput quantification of diverse microbial risks in the environment. Current metagenomic next-generation sequencing approaches can achieve high-throughput detection of genes indicative of microbial risks but lack quantitative capabilities. This study developed and tested a quantitative metagenomic next-generation sequencing (qmNGS) approach. Numerous xenobiotic synthetic internal DNA standards were used to determine the sequencing yield (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.