Evidence map›Paper›PMID 34085242›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification Sequencing.

Bryan R Cullen, Kevin Tsai

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. RNA modifications and their role in gene expression.Frontiers in molecular biosciences · 2025
    Review
  3. When animal viruses meet NVeterinary research · 2024
    Review
  4. Article
  5. Multiple roles of mMolecular biology reports · 2022
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Bryan R CullenDepartment of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA.
Kevin TsaiDepartment of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA. kevtsai@ibms.sinica.edu.tw.
Duke Medical Center · USDuke University Hospital · US

Funding

Social and Behavioral Sciences CoreP30AI064518 · NIAID · DUKE UNIVERSITY · PI Nwora Lance Okeke · 2005 to 2026
$50.5M
The Center for HIV RNA Studies (CRNA)P50GM103297 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAYIE, KWAKU · 2012 to 2016
$21.7M
The Center for HIV RNA Studies (CRNA)U54AI150470 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TOLBERT, BLANTON SMITH · 2019 to 2021
$15.1M
The Center for HIV RNA Studies (CRNA)U54GM103297 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TELESNITSKY, ALICE · 2017 to 2018
$10.2M
Epitranscriptomic modification of HIV-1 transcripts: Effects of drugs of abuseR01DA046111 · NIDA · DUKE UNIVERSITY · PI CULLEN, BRYAN R. · 2018 to 2022
$1.6M
NIAID NIH HHS P30 AI064518NIAID NIH HHS U54 AI150470NIDA NIH HHS R01 DA046111NIGMS NIH HHS P50 GM103297NIGMS NIH HHS U54 GM103297
6 · The paper itself

Abstract

Epitranscriptomic RNA modifications function as an important layer of gene regulation that modulates the function of RNA transcripts. A key step in understanding how RNA modifications regulate biological processes is the mapping of their locations, which is most commonly done by RNA immunoprecipitation (RIP) using modification-specific antibodies. Here, we describe the use of a photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP) method, in conjunction with RNA modification-specific antibodies, to map modification sites. First described as photo-crosslinking-assisted m

Indexed as

Gene Expression ProfilingHumansImmunoprecipitationRibonucleosidesRNARNA Processing, Post-TranscriptionalSequence Analysis, RNATranscriptomeRibonucleosidesRNAac4CEpitranscriptomic RNA modificationm5Cm6ARNA immunoprecipitation

Identifiers

PMID34085242
PMCPMC8259884
OpenAlexW3164636380

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.