Evidence map›Paper›PMID 34085193›Full record

ArticleMolecular biology reports2021

Urinary exosomal microRNA-96-5p and microRNA-183-5p expression as potential biomarkers of bladder cancer.

Amal S El-Shal, Sally M Shalaby, Safwat E Abouhashem, Eman H Abd Elbary, Samir Azazy, Nearmeen M Rashad, Walaa Sarhan

Abstract readMulticenter Study
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 6 pooled it
6.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 6 syntheses or guidelines pooled it, 39 citations in OpenAlex.

  1. Pooled it
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  3. Exosomal noncoding RNAs as noninvasive biomarkers in bladder cancer: a diagnostic meta-analysis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2024
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  7. NiClJournal of fluorescence · 2026
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  19. Circulating exosomal miRNAs and cancer early diagnosis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Amal S El-ShalMedical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Sally M ShalabyMedical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. sallyshalaby@hotmail.com.ORCID http://orcid.org/0000-0002-2868-020X
Safwat E AbouhashemUrology Departments, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Eman H Abd ElbaryPathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Samir AzazyUrology Department, Faculty of Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Nearmeen M RashadInternal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Walaa SarhanMedical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Zagazig University · EGAin Shams University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Because of low sensitivity and specificity of the currently available urine biomarkers of bladder cancer (BC) detection and painful cystoscopy procedure. Our study aimed to evaluate expression of urinary exosomal miR-96-5p and miR-183-5p as probable non-invasive and accurate biomarkers for the diagnosis and follow up of BC. Using quantitative real-time polymerase chain reaction; expression of exosomal microRNA (miR)-96-5p and miR- 183-5p in the urine samples of 51 patients with BC, 21 patients with benign urinary bladder lesions and in 24 normal individuals as control group was done. Our study results showed higher expressions of both miR-96-5p and miR-183-5p in urine of BC patients in comparison with control group (P < 0.001 for each). Receiver operating characteristic curve (ROC) analysis showed that each microRNA had good sensitivity and specificity to differentiate BC from non-BC patients miR-96-5p 80.4% and 91.8% and miR-183-5p 78.4% and 81.6% respectively compared to cytology (37.3% and 100%). In addition, it was obvious that the sensitivity of combined miR-96-5p and miR-183-5p for the diagnosis of BC reached 88.2%% and specificity reached 87.8%, which were higher than each one alone. We also found that expression of miR-96-5p and miR-183-5p with high grade, and pathological stage was significantly increased. After surgery, collected urine samples showed significantly lower expression of miR-96-5p-: P < 0.001; and miR-183-5p: P = 0.002. In conclusion, urine miR-96-5p and miR-183-5p are promising tumor biomarkers of BC diagnosis; particularly, when they combined with each other or with urinary cytology.

Indexed as

Gene ExpressionGene Expression Regulation, NeoplasticBiomarkers, TumorCase-Control StudiesExosomesFemaleHumansMaleMicroRNAsMiddle AgedPrognosisProspective StudiesReal-Time Polymerase Chain ReactionROC CurveSensitivity and SpecificityUrinary Bladder NeoplasmsBiomarkers, TumorMicroRNAsMIRN183 microRNA, humanMIRN96 microRNA, humanBiochemical markerBladder cancerExosomalmiRNA-183miRNA-96Real-time PCR

Identifiers

PMID34085193
OpenAlexW3138332844

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.