ArticleChemical science2019
Single-molecule nanopore sensing of actin dynamics and drug binding.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Catalytic Proximal Protein Oligomerization as an Anti-Tumor Strategy Targeting WDR5.Nature communications · 2026Article
- MR Imaging Reveals Dynamic Aggregation of Multivalent Glycoconjugates in Aqueous Solution.Inorganic chemistry · 2024Article
- Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules.Biosensors · 2024Review
- Selective Single-Molecule Nanopore Detection of mpox A29 Protein Directly in Biofluids.Nano letters · 2023Article
- Single-Molecule Detection of α-Synuclein Oligomers in Parkinson's Disease Patients Using Nanopores.ACS nano · 2023Article
- Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes.Nature communications · 2023Article
- Nanopore Detection Using Supercharged Polypeptide Molecular Carriers.Journal of the American Chemical Society · 2023Article
- Biohybrid Membrane Formation by Directed Insertion of Aquaporin into a Solid-State Nanopore.ACS applied materials & interfaces · 2022Article
- Single-Molecule Binding Assay Using Nanopores and Dimeric NP Conjugates.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum.Nature communications · 2021Article
- The Diagnostic Potential of Amyloidogenic Proteins.International journal of molecular sciences · 2021Review
- Modulation of electrophoresis, electroosmosis and diffusion for electrical transport of proteins through a solid-state nanopore.RSC advances · 2021Article
- Macromolecular Crowding Enhances the Detection of DNA and Proteins by a Solid-State Nanopore.Nano letters · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
Actin is a key protein in the dynamic processes within the eukaryotic cell. To date, methods exploring the molecular state of actin are limited to insights gained from structural approaches, providing a snapshot of protein folding, or methods that require chemical modifications compromising actin monomer thermostability. Nanopore sensing permits label-free investigation of native proteins and is ideally suited to study proteins such as actin that require specialised buffers and cofactors. Using nanopores, we determined the state of actin at the macromolecular level (filamentous or globular) and in its monomeric form bound to inhibitors. We revealed urea-dependent and voltage-dependent transitional states and observed the unfolding process within which sub-populations of transient actin oligomers are visible. We detected, in real-time, filament-growth, and drug-binding at the single-molecule level demonstrating the promise of nanopore sensing for in-depth understanding of protein folding landscapes and for drug discovery.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.