Evidence map›Paper›PMID 34082708›Full record

ArticleBMC genomics2021

Transcriptional dynamics of transposable elements when converting fibroblast cells of Macaca mulatta to neuroepithelial stem cells.

Dahai Liu, Li Liu, Kui Duan, Junqiang Guo, Shipeng Li, Zhigang Zhao, Xiaotuo Zhang, Nan Zhou, Yun Zheng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Dahai Liu *Foshan Stomatology Hospital and School of Medicine, Foshan University, Foshan, Guangdong, 528000, China.
Li LiuState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
Kui DuanState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
Junqiang GuoFaculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
Shipeng LiState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
Zhigang ZhaoState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
Xiaotuo ZhangState Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Nan ZhouFaculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
Yun ZhengState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China. zhengyun5488@gmail.com.ORCID http://orcid.org/0000-0003-4292-9806
Kunming University of Science and Technology · CNFoshan University · CNFudan University · CN

Funding

Department of Education of Guangdong Province 2017KTSCX189Ministry of Science and Technology of China 2018YFA0108502National Natural Science Foundation of China 31760314National Natural Science Foundation of China 81570376National Natural Science Foundation of China 81870307
6 · The paper itself

Abstract

backgroundTransposable elements (TE) account for more than 50% of human genome. It has been reported that some types of TEs are dynamically regulated in the reprogramming of human cell lines. However, it is largely unknown whether some TEs in Macaca mulatta are also regulated during the reprogramming of cell lines of monkey.

resultsHere, we systematically examined the transcriptional activities of TEs during the conversion of Macaca mulatta fibroblast cells to neuroepithelial stem cells (NESCs). Hundreds of TEs were dynamically regulated during the reprogramming of Macaca mulatta fibroblast cells. Furthermore, 48 Long Terminal Repeats (LTRs), as well as some integrase elements, of Macaca endogenous retrovirus 3 (MacERV3) were transiently activated during the early stages of the conversion process, some of which were further confirmed with PCR experiments. These LTRs were potentially bound by critical transcription factors for reprogramming, such as KLF4 and ETV5.

conclusionThese results suggest that the transcription of TEs are delicately regulated during the reprogramming of Macaca mulatta fibroblast cells. Although the family of ERVs activated during the reprogramming of fibroblast cells in Macaca mulatta is different from those in the reprogramming of human fibroblast cells, our results suggest that the activation of some ERVs is a conserved mechanism in primates for converting fibroblast cells to stem cells.

Indexed as

DNA Transposable ElementsTerminal Repeat SequencesAnimalsFibroblastsHumansKruppel-Like Factor 4Macaca mulattaStem CellsDNA Transposable ElementsKLF4 protein, humanKruppel-Like Factor 4Endogenous retrovirus (ERV)FibroblastInduced pluripotent stem cell (iPSC)Long terminal repeat (LTR)Neuroepithelial stem cell (NESC)TranscriptionTransposable element (TE)

Identifiers

PMID34082708
PMCPMC8176597
OpenAlexW3163965606

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.