Evidence map›Paper›PMID 34081250›Full record

ArticleDigestive diseases and sciences2022

LGR5-Expressing Cells in the Healing Process of Post-ESD Ulcers in Gastric Corpus.

Yosuke Tobe, Takeshi Uehara, Tomoyuki Nakajima, Mai Iwaya, Yukihiro Kobayashi, Yasuhiro Kinugawa, Yasuhiro Kuraishi, Hiroyoshi Ota

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Article in Digestive diseases and sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yosuke TobeDepartment of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Takeshi UeharaDepartment of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan. tuehara@shinshu-u.ac.jp.ORCID 0000-0002-7694-9015
Tomoyuki NakajimaDepartment of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Mai IwayaDepartment of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Yukihiro KobayashiDepartment of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Yasuhiro KinugawaDepartment of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Yasuhiro KuraishiDepartment of Gastroenterology, Shinshu University School of Medicine, Matsumoto, Japan.
Hiroyoshi OtaDepartment of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Shinshu University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLGR5 is a promising stem cell marker in gastric pylorus, but there are few reports on its expression in human gastric corpus.

aimsTo investigate the involvement of LGR5 expression in gastric corpus ulcer regeneration in humans.

methodsLGR5 expression was analyzed in five post-ESD ulcers during the healing process of regenerating epithelial cells of the gastric corpus. LGR5 expression was detected by mRNA in situ hybridization using an RNA scope kit. Immunohistochemistry of MUC6, HIK1083, and pepsinogen 1 (PG1) was performed to identify cell differentiation.

resultsWe defined MUC6+/HIK1083-/PG1-, MUC6+/HIK1083+/PG1-, MUC6+/HIK1083+/PG1+, MUC6+/HIK1083-/PG1+, and MUC6-/HIK1083-/PG1+cells as pseudopyloric mucosa (PPM) phase 1 (PPM1), PPM phase 2 (PPM2), PPM phase 3 (PPM3), immature chief cells (ICC), and mature chief cells (MCC) in order from the ulcer center, respectively. In the regenerated mucosa around post-ESD ulcers, LGR5 expression was observed throughout the gland in PPM1-PPM3, but it was limited to the bottom of the gland in ICC and MCC. Furthermore, LGR5 expression was not identified in the normal gastric corpus. The H-score of PPM2 was significantly higher than that of PPM3 (P = 0.0313). The H-score of PPM3 was significantly higher than that of ICC (P = 0.0313). The LGR5 H-score was higher at the immature stage, which decreased gradually with progression of the differentiation stage.

conclusionsLGR5 expression appears to contribute to mucosal regeneration in the human gastric corpus. The application of LGR5 expression analysis to mucosal regeneration and fundic gland-type gastric tumors is expected.

Indexed as

Stomach NeoplasmsStomach UlcerGastric MucosaHumansImmunohistochemistryReceptors, G-Protein-CoupledUlcerLGR5 protein, humanReceptors, G-Protein-CoupledChief cellLGR5Pseudopyloric metaplasiaRNA in situ hybridization

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PMID34081250
OpenAlexW3164512569

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.