ArticleThe journal of physical chemistry letters2021
Identification of Thioflavin T Binding Modes to DNA: A Structure-Specific Molecular Probe for Lasing Applications.
Article in The journal of physical chemistry letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
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- Targeting a genomic RNA G-quadruplex of dengue virus with small molecules as an alternative to protein-targeted therapeutics.Journal of biomedical science · 2026Article
- Capture-SELEX of DNA Aptamers for Highly Selective Binding of Folate.Chembiochem : a European journal of chemical biology · 2026Article
- DNA Modulates Structural Transitions and Oligomerization Kinetics of the Functional Amyloid CRES.Journal of the American Chemical Society · 2026Article
- Phosphorylation event changes the RNA binding mode of EZH2 disordered segment.Protein science : a publication of the Protein Society · 2026Article
- Transport Properties of Self-Assembling G-Hydrogels: Evidence for a Tunable Fickian Diffusivity.The journal of physical chemistry. B · 2025Article
- Biomimetic Silk Nanoparticle Manufacture: Calcium Ion-Mediated Assembly.ACS biomaterials science & engineering · 2025Article
- Recent Advances in DNA Origami-Enabled Optical Biosensors for Multi-Scenario Application.Nanomaterials (Basel, Switzerland) · 2024Review
- Role of Alkali Cations in DNA-Thioflavin T Interaction.The journal of physical chemistry. B · 2024Article
- G-quadruplex formation in RNA aptamers selected for binding to HIV-1 capsid.Frontiers in chemistry · 2024Article
- The potential of fluorogenicity for single molecule FRET and DyeCycling.QRB discovery · 2024Review
- Cavity Lasing Characteristics of Thioflavin T and Thioflavin X in Different Solvents and Their Interaction with DNA for the Controlled Reduction of a Light Amplification Threshold in Solid-State Biofilms.ACS applied optical materials · 2023Article
- The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast Saccharomyces cerevisiae.Bioscience reports · 2023Article
- Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence.Gels (Basel, Switzerland) · 2023Article
- Switching G-quadruplex to parallel duplex by molecular rotor clustering.Nucleic acids research · 2022Article
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4 authors.
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Abstract
The binding mechanism of thioflavin T (ThT) to DNA was studied using polarized light spectroscopy and fluorescence-based techniques in solutions and in solid films. Linear dichroism measurements showed that ThT binds to DNA duplex by intercalation. Time-resolved fluorescence studies revealed a second binding mode which is the external binding to the DNA phosphate groups. Both binding modes represent the nonspecific type of interactions. The studies were complemented with the analysis of short oligonucleotides having DNA cavities. The results indicate that the interplay between three binding modes-intercalation, external binding, and binding inside DNA cavities-determines the effective fluorescence quantum yield of the dye in the DNA structures. External binding was found to be responsible for fluorescence quenching because of energy transfer between intercalated and externally bound molecules. Finally, amplified spontaneous emission (ASE) was successfully generated in the ThT-stained films and used for detecting different DNA structures. ASE measurements show that ThT-stained DNA structures can be used for designing bioderived microlasers.
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