Evidence map›Paper›PMID 34079033›Full record

ArticleScientific reports2021

Human umbilical cord mesenchymal stem cell-derived extracellular vesicles attenuate experimental autoimmune encephalomyelitis via regulating pro and anti-inflammatory cytokines.

Samira Ahmadvand Koohsari, Abdorrahim Absalan, Davood Azadi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 2 pooled it
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.

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  10. Stem cell and gene therapies for leukodystrophies.Molecular therapy. Methods & clinical development · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Samira Ahmadvand KoohsariDepartment of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
Abdorrahim AbsalanDepartment of Medical Laboratory Sciences, Khomein University of Medical Sciences, Ghods Avenue, Khomein, Markazi Province, 38818-58573, Iran. a.r.absalan@gmail.com.
Davood AzadiDepartment of Medical Laboratory Sciences, Khomein University of Medical Sciences, Ghods Avenue, Khomein, Markazi Province, 38818-58573, Iran.
Imam Khomeini Hospital · IRIslamic Azad University, Marvdasht · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The therapeutic effects of mesenchymal stem cells-extracellular vesicles have been proved in many inflammatory animal models. In the current study, we aimed to investigate the effect of extracellular vesicles (EVs) derived from human umbilical cord-MSC (hUCSC-EV) on the clinical score and inflammatory/anti-inflammatory cytokines on the EAE mouse model. After induction of EAE in C57Bl/6 mice, they were treated intravenously with hUCSC-EV or vehicle. The clinical score and body weight of all mice was registered every day. On day 30, mice were sacrificed and splenocytes were isolated for cytokine assay by ELISA. Cytokine expression of pro-/anti-inflammatory cytokine by real-time PCR, leukocyte infiltration by hematoxylin and eosin (H&E) staining, and the percent of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) positive cells by immunohistochemistry were assessed in the spinal cord. Our results showed that hUCSC-EV-treated mice have lower maximum mean clinical score (MMCS), pro-inflammatory cytokines, and inflammatory score in comparison to the control mice. We also showed that hUCSC-EV administration significantly improved body weight and increased the anti-inflammatory cytokines and the frequency of Treg cells in the spleen. There was no significant difference in the percent of GFAP and MBP positive cells in the spinal cord of experimental groups. Finally, we suggest that intravenous administration of hUCSC-EV alleviate induce-EAE by reducing the pro-inflammatory cytokines, such as IL-17a, TNF-α, and IFN-γ, and increasing the anti-inflammatory cytokines, IL-4 and IL-10, and also decrease the leukocyte infiltration in a model of MS. It seems that EVs from hUC-MSCs have the same therapeutic effects similar to EVs from other sources of MSCs, such as adipose or bone marrow MSCs.

Indexed as

AnimalsBody WeightEncephalomyelitis, Autoimmune, ExperimentalExtracellular VesiclesFemaleFetal BloodGene Expression RegulationGlial Fibrillary Acidic ProteinHumansInterferon-gammaInterleukin-10Interleukin-17Interleukin-4Mesenchymal Stem CellsMiceMice, Inbred C57BLGlial Fibrillary Acidic Proteinglial fibrillary astrocytic protein, mouseIFNG protein, mouseIL10 protein, mouseIl17a protein, mouseInterferon-gammaInterleukin-10Interleukin-17Interleukin-4Mbp protein, mouseMyelin Basic ProteinTumor Necrosis Factor-alpha

Identifiers

PMID34079033
PMCPMC8172573
OpenAlexW3165026729

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.