ArticleScientific reports2021
Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- In vitro production significantly reduces metabolic differences among bovine embryos.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Effects of L-Carnitine on the Developmental Competence of Bovine Oocytes.Animals : an open access journal from MDPI · 2025Article
- Bovine Endometrium Drives and Responds to Divergence of In Vitro Produced Conceptus Biochemistry.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Supplementation of Forskolin and Linoleic Acid During IVC Improved the Developmental and Vitrification Efficiency of Bovine Embryos.International journal of molecular sciences · 2025Article
- Factors Affecting the Success of Ovum Pick-Up, In Vitro Production and Cryopreservation of Embryos in Cattle.Animals : an open access journal from MDPI · 2025Review
- Oviduct epithelial spheroids during in vitro culture of bovine embryos mitigate oxidative stress, improve blastocyst quality and change the embryonic transcriptome.Biological research · 2024Article
- Different responses of larval fatty acid profiles to cryopreservation in two commercially important bivalves.Scientific reports · 2024Article
- Review
- The recipient metabolome explains the asymmetric ovarian impact on fetal sex development after embryo transfer in cattle.Journal of animal science · 2024Article
- Impact of antral follicle count on follicular-luteal characteristics, superovulatory response, and embryo quality in Sahiwal cows.Frontiers in veterinary science · 2024Article
- Associations of antral follicle count with fertility in cattle: A review.JDS communications · 2023Review
- Species and embryo genome origin affect lipid droplets in preimplantation embryos.Frontiers in cell and developmental biology · 2023Article
- The proteomic analysis of bovine embryos developed in vivo or in vitro reveals the contribution of the maternal environment to early embryo.BMC genomics · 2022Article
- Non-Invasive Identification of Sex in Cultured Bovine Embryos by UHPLC-MS/MS Metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2022Article
- Effects of the donor factors and freezing protocols on the bovine embryonic lipid profile†.Biology of reproduction · 2022Article
- Dynamic Changes in the Proteome of Early Bovine Embryos DevelopedFrontiers in cell and developmental biology · 2022Article
- The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer.Metabolites · 2021Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with lipid accumulation in the cytoplasm mainly due to in vitro culture conditions. The objective of this study was to evaluate the lipid composition of biopsied and sexed embryos, produced either in vivo or in vitro from the same Holstein heifers before and after a slow freezing protocol. Lipid extracts were analysed by liquid chromatography-high resolution mass spectrometry, which enabled the detection of 496 features. Our results highlighted a lipid enrichment of IVP embryos in triglycerides and oxidised glycerophospholipids and a reduced abundance in glycerophospholipids. The slow freezing process affected the lipid profiles of IVP and IVD embryos similarly. Lysophosphatidylcholine content was reduced when embryos were frozen/thawed. In conclusion, the embryonic lipid profile is impacted by IVP and slow freezing protocols but not by sex. Lysophosphatidylcholine seemed highly sensitive to cryopreservation and might contribute to explain the lower quality of frozen embryos. Further studies are required to improve embryo freezability by modulating the lipidome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.