ArticleNature communications2021
Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 101 citations in OpenAlex.
- The notochord: development, disease and stem cell-based modelling.Development (Cambridge, England) · 2026Review
- Optogenetic WNT signaling drives germ layer self-organization in a human gastruloid model.bioRxiv : the preprint server for biology · 2026Article
- BMP4 initiates and patterns ventral-caudal structures in zebrafish and human pluripotent stem cell aggregates.The EMBO journal · 2026Article
- Organizer activity in the mouse embryo.Cells & development · 2025Review
- Transgene-free mouse embryo models from chemical reprogramming reach early organogenesis.Cell regeneration (London, England) · 2025Article
- Stem Cell and Synthetic Embryo Models: Advances, Applications, and Ethical Considerations.Stem cell reviews and reports · 2025Review
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- Signaling Centers Drive Brachyury Dynamics and Lineage Commitment in mESC Aggregates.FASEB bioAdvances · 2025Article
- Article
- In vitro modelling of anterior primitive streak patterning with human pluripotent stem cells identifies the path to notochord progenitors.Development (Cambridge, England) · 2024Article
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Engineering a computable epiblast for in silico modeling of developmental toxicity.Reproductive toxicology (Elmsford, N.Y.) · 2024Article
- Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis.Nature biotechnology · 2024Article
- Article
- Article
- Robust axis elongation by Nodal-dependent restriction of BMP signaling.Development (Cambridge, England) · 2024Article
- Topical section: embryonic models (2023) for Current Opinion in Genetics & Development.Current opinion in genetics & development · 2024Review
- Generation of Stem Cell-Based Mouse Embryo-Like Structures.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Gastruloids - a minimalistic model to study complex developmental metabolism.Emerging topics in life sciences · 2023Review
- Signaling mechanisms that direct cell fate specification and morphogenesis in human embryonic stem cells-based models of human gastrulation.Emerging topics in life sciences · 2023Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generating properly differentiated embryonic structures in vitro from pluripotent stem cells remains a challenge. Here we show that instruction of aggregates of mouse embryonic stem cells with an experimentally engineered morphogen signalling centre, that functions as an organizer, results in the development of embryo-like entities (embryoids). In situ hybridization, immunolabelling, cell tracking and transcriptomic analyses show that these embryoids form the three germ layers through a gastrulation process and that they exhibit a wide range of developmental structures, highly similar to neurula-stage mouse embryos. Embryoids are organized around an axial chordamesoderm, with a dorsal neural plate that displays histological properties similar to the murine embryo neuroepithelium and that folds into a neural tube patterned antero-posteriorly from the posterior midbrain to the tip of the tail. Lateral to the chordamesoderm, embryoids display somitic and intermediate mesoderm, with beating cardiac tissue anteriorly and formation of a vasculature network. Ventrally, embryoids differentiate a primitive gut tube, which is patterned both antero-posteriorly and dorso-ventrally. Altogether, embryoids provide an in vitro model of mammalian embryo that displays extensive development of germ layer derivatives and that promises to be a powerful tool for in vitro studies and disease modelling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.