Evidence map›Paper›PMID 34078310›Full record

ArticleBMC cancer2021

LINC_00355 promotes gastric cancer progression by upregulating PHF19 expression through sponging miR-15a-5p.

Jishui Zhang, Wenhao Lv, Yagang Liu, Weihua Fu, Baosheng Chen, Qiutong Ma, Xin Gao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Insights into high-risk multiple myeloma from an analysis of the role of PHF19 in cancer.Journal of experimental & clinical cancer research : CR · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jishui ZhangDepartment of Gastrointestinal Surgery, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, 061001, Hebei, China. jishui_z1980@163.com.ORCID http://orcid.org/0000-0002-5573-7988
Wenhao LvDepartment of Gastrointestinal Surgery, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, 061001, Hebei, China.
Yagang LiuDepartment of Gastrointestinal Surgery, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, 061001, Hebei, China.
Weihua FuDepartment of General Surgery, Tianjin Medical University General Hospital, Tianjin, 300052, China.
Baosheng ChenDepartment of Gastrointestinal Surgery, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, 061001, Hebei, China.
Qiutong MaDepartment of Gastrointestinal Surgery, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, 061001, Hebei, China.
Xin GaoDepartment of Radiotherapy, Cangzhou Central Hospital, Cangzhou, 061001, Hebei, China.
Cangzhou Central Hospital · CNTianjin Medical University General Hospital · CN

Funding

the Medical Science Research Program of Health Commission of Hebei Province No. 20200174
6 · The paper itself

Abstract

backgroundLong non-coding RNAs exert vital roles in several types of cancer. The objective of this study was to explore the role of LINC_00355 in gastric cancer (GC) progression and its potential mechanism.

methodsThe expression levels of LINC_00355 in GC tissues and cells were detected by quantitative real-time PCR, followed by assessing the effects of LINC_00355 knockdown or overexpression on cell properties. Dual-luciferase reporter assay was utilized to identify the relationship between LINC_00355 and microRNA (miR)-15a-5p and miR-15a-5p and PHD finger protein 19 (PHF19), followed by the rescue experiments.

resultsThe results showed that LINC_00355 was highly expressed in GC tissues and cells compared with the corresponding control. LINC_00355 knockdown decreased the viability, migration, and invasion and increased the accumulation of GC cells in G1 phase and apoptosis. Meanwhile, LINC_00355 downregulation markedly increased cleaved caspase 3 and cleaved poly (ADP-ribose) polymerase protein levels, whereas decreased cyclin D1, cyclin E, matrix metalloproteinase (MMP) 9, MMP2, and N-cadherin protein levels in GC cells. However, LINC_00355 overexpression had the opposite effects. It was verified that LINC_00355 upregulated the expression of PHF19 through sponging miR-15a-5p. Furthermore, PHF19 overexpression reversed the effect of LINC_00355 knockdown on GC cell properties, including cell viability, migration, invasion, and apoptosis.

conclusionsCollectively, these results suggest that LINC_00355 promotes GC progression by up-regulating PHF19 through sponging miR-15a-5p. Our findings may provide an important clinical basis for reversing the malignant phenotype of GC.

Indexed as

Gene Expression Regulation, NeoplasticApoptosisBiopsyCell Line, TumorDNA-Binding ProteinsG1 Phase Cell Cycle CheckpointsGastric MucosaGene Knockdown TechniquesHumansMicroRNAsRNA, Long NoncodingStomach NeoplasmsTranscriptional ActivationTranscription FactorsUp-RegulationDNA-Binding ProteinsMicroRNAsMIRN15 microRNA, humanPHF19 protein, humanRNA, Long NoncodingTranscription FactorsGastric cancerLINC_00355miR-15a-5pPHD finger protein 19

Identifiers

PMID34078310
PMCPMC8170819
OpenAlexW3165294311

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.