Evidence map›Paper›PMID 34077426›Full record

ArticlePloS one2021

PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.

Arunkumar Arumugam, Cole Markham, Saurabh S Aykar, Barbara Van Der Pol, Paula Dixon, Michelle Wu, Season Wong

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Influence of COAntibiotics (Basel, Switzerland) · 2024
    Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Arunkumar ArumugamAI Biosciences, Inc., College Station, Texas, United States of America.
Cole MarkhamAI Biosciences, Inc., College Station, Texas, United States of America.
Saurabh S AykarIowa State University, Ames, Iowa, United States of America.
Barbara Van Der PolUniversity of Alabama at Birmingham, Birmingham, Alabama, United States of America.
Paula DixonUniversity of Alabama at Birmingham, Birmingham, Alabama, United States of America.
Michelle WuAI Biosciences, Inc., College Station, Texas, United States of America.
Season WongAI Biosciences, Inc., College Station, Texas, United States of America.ORCID 0000-0001-5264-0286
AI Biosciences (United States) · USUniversity of Alabama at Birmingham · USIowa State University · US

Funding

POINT OF CARE DIAGNOSTIC FOR GONORRHEA AND DETERMINATION OF ANTIMICROBIAL SUSCEPTIBILITY75N93019C00018 · NIAID · AI BIOSCIENCES, INC. · PI WONG, SEASON · 2019 to 2019
$300k
NIAID NIH HHS 75N93019C00018
6 · The paper itself

Abstract

Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (< $400), open-source CO2 incubator. The system is comprised of a Raspberry Pi computer connected to a 3D printer controller board that has controls for a CO2 sensor, solenoid valve, heater, and thermistors. CO2 is supplied through the sublimation of dry ice stored inside a thermos to create a sustained 5% CO2 supply. The unit is controlled via G-Code commands sent by the Raspberry Pi to the controller board. In addition, we built a custom software application for remote control and used the open-source Grafana dashboard for remote monitoring. Our data show that we can maintain consistent CO2 and temperature levels for over three days without manual interruption. The results from our culture plates and real-time PCR indicate that our incubator performed equally well when compared to a much more expensive commercial CO2 incubator. We have also demonstrated that the antibiotic susceptibility assay can be performed in this low-cost CO2 incubator. Our work also indicates that the system can be connected to incubator chambers of various chamber volumes.

Indexed as

Anti-Bacterial AgentsCarbon DioxideGonorrheaHumansIncubatorsNeisseria gonorrhoeaePrinting, Three-DimensionalSoftwareAnti-Bacterial AgentsCarbon Dioxide

Identifiers

PMID34077426
PMCPMC8172042
OpenAlexW3164132477

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.