ArticleThe New phytologist2021
An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Optimization of selection agent concentrations and expanding G418 utility for gentamicin resistance in Marchantia polymorpha.Scientific reports · 2025Article
- A Simple and Scalable Chopped-Thallus Transformation Method forPlants (Basel, Switzerland) · 2025Article
- The transcription factor PpRKD evokes female developmental fate in the sexual reproductive organs of Physcomitrium patens.The New phytologist · 2025Article
- Pan-phylum genomes of hornworts reveal conserved autosomes but dynamic accessory and sex chromosomes.Nature plants · 2025Article
- A simple, highly efficientaBIOTECH · 2024Article
- The role of fossils for reconstructing the evolution of plant development.Development (Cambridge, England) · 2024Article
- ΔiScience · 2024Article
- An optimized transformation protocol for Anthoceros agrestis and three more hornwort species.The Plant journal : for cell and molecular biology · 2023Article
- Unique opportunities for future research on the alternative oxidase of plants.Plant physiology · 2023Article
- What can hornworts teach us?Frontiers in plant science · 2023Review
- Evolution of meristem zonation by CLE gene duplication in land plants.Nature plants · 2022Review
- The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants.The Plant cell · 2022Review
- Molecular biology of mosses.Plant molecular biology · 2021Article
- Step-by-step protocol for the isolation and transient transformation of hornwort protoplasts.Applications in plant sciencesArticle
- Accelerating gametophytic growth in the model hornwortApplications in plant sciencesArticle
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12 authors.
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Abstract
Despite their key phylogenetic position and their unique biology, hornworts have been widely overlooked. Until recently there was no hornwort model species amenable to systematic experimental investigation. Anthoceros agrestis has been proposed as the model species to study hornwort biology. We have developed an Agrobacterium-mediated method for the stable transformation of A. agrestis, a hornwort model species for which a genetic manipulation technique was not yet available. High transformation efficiency was achieved by using thallus tissue grown under low light conditions. We generated a total of 274 transgenic A. agrestis lines expressing the β-glucuronidase (GUS), cyan, green, and yellow fluorescent proteins under control of the CaMV 35S promoter and several endogenous promoters. Nuclear and plasma membrane localization with multiple color fluorescent proteins was also confirmed. The transformation technique described here should pave the way for detailed molecular and genetic studies of hornwort biology, providing much needed insight into the molecular mechanisms underlying symbiosis, carbon-concentrating mechanism, RNA editing and land plant evolution in general.
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