ArticleThe Analyst2021
Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing.
Article in The Analyst, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- A self-heating, multi-channel slider cassette for innovative point-of-care molecular diagnostics from whole blood samples.Biosensors & bioelectronics · 2025Article
- 3D-Printed CRISPR-based detection system powered by a reusable handwarmer.Biomedical microdevices · 2025Article
- Smartphone-Linked and Electricity-Free Platforms for Rapid Colorimetric Molecular Detection of Poultry Respiratory Viruses at the Point of Need.Biosensors · 2025Article
- Chemical Heating for Minimally Instrumented Point-of-Care (POC) Molecular Diagnostics.Biosensors · 2024Review
- Review
- An Ultra-Compact and Low-Cost LAMP-Based Virus Detection Device.Sensors (Basel, Switzerland) · 2024Article
- A Colorimetric RT-LAMP Assay for Rapid Detection ofACS omega · 2024Article
- An overview for monitoring and prediction of pathogenic microorganisms in the atmosphere.Fundamental research · 2024Review
- Article
- Maximising Affordability of Real-Time Colorimetric LAMP Assays.Micromachines · 2023Article
- Rapid and Sensitive Diagnosis of COVID-19 Using an Electricity-Free Self-Testing System.Biosensors · 2023Article
- MINI: A high-throughput point-of-care device for performing hundreds of nucleic acid tests per day.Biosensors & bioelectronics · 2022Article
- Manually-Operated, Slider Cassette for Multiplexed Molecular Detection at the Point of Care.Sensors and actuators. B, Chemical · 2022Article
- Rapid and simple detection of influenza virus via isothermal amplification lateral flow assay.Analytical and bioanalytical chemistry · 2022Article
- Portable Electrochemical DNA Sensors Based on Gene Amplification Reactions to Screen and Identify Pathogen and SNPs.Sensors (Basel, Switzerland) · 2022Article
- Sensitive, Single-Pot, Two-Stage Assay for Hepatitis Viruses.Analytical chemistry · 2022Article
- Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip.Animals : an open access journal from MDPI · 2021Article
- Single- and Two-Stage, Closed-Tube, Point-of-Care, Molecular Detection of SARS-CoV-2.Analytical chemistry · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Molecular detection of pathogenic nucleic acids from patient samples requires incubating biochemical reactions at specific temperatures to amplify DNA. This incubation is typically carried out with an electrical heater and a temperature controller. To reduce test cost, to eliminate the need for manufacturing incubators, which may require significant time, and to enable electricity-free operation, we use energetic compounds such as an Mg(Fe) alloy mixed with a phase-change material (PCM) that undergoes phase transformation at the desired incubation temperature. We dubbed this composite Energetic Phase Change Material (EPCM). When the EPCM is brought into contact with water, the magnesium alloy interacts with the water to produce heat. The EPCM heats up to its phase transition temperature. Any excess heat is absorbed as latent heat and the system is maintained at its desired incubation temperature, independent of ambient temperatures, long enough to facilitate enzymatic amplification. The EPCM together with colorimetric amplicon detection facilitates an inexpensive, disposable, point-of-need diagnostic test that does not require any electric power. We demonstrate the feasibility of our approach by detecting SARS-Cov-2 in saliva samples either without any instrumentation or with a palm-size CCD camera that enables us to follow the amplification process in real time.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.