Evidence map›Paper›PMID 34075906›Full record

ArticleBiomaterials science2021

TCF-ALP: a fluorescent probe for the selective detection of

Lauren Gwynne, George T Williams, Kai-Cheng Yan, Bethany L Patenall, Jordan E Gardiner, Xiao-Peng He, Jean-Yves Maillard, Tony D James, Adam C Sedgwick, A Toby A Jenkins

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 4 countries.

Lauren GwynneDepartment of Chemistry, University of Bath, BA2 7AY, UK. a.t.a.jenkins@bath.ac.uk.ORCID http://orcid.org/0000-0001-7690-5947
George T WilliamsDepartment of Chemistry, University of Bath, BA2 7AY, UK. a.t.a.jenkins@bath.ac.uk and School of Physical sciences, University of Kent, CT2 7NH, UK.ORCID http://orcid.org/0000-0001-6162-8895
Kai-Cheng YanKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, Frontiers Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.ORCID http://orcid.org/0000-0001-8869-7217
Bethany L PatenallDepartment of Chemistry, University of Bath, BA2 7AY, UK. a.t.a.jenkins@bath.ac.uk.ORCID http://orcid.org/0000-0001-9042-4744
Jordan E GardinerDepartment of Chemistry, University of Bath, BA2 7AY, UK. a.t.a.jenkins@bath.ac.uk.ORCID http://orcid.org/0000-0003-0349-6614
Xiao-Peng HeKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, Frontiers Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.ORCID http://orcid.org/0000-0002-8736-3511
Jean-Yves MaillardSchool of Pharmacy and Pharmaceutical Sciences, Cardiff University, CF10 3NB, UK.ORCID http://orcid.org/0000-0002-8617-9288
Tony D JamesDepartment of Chemistry, University of Bath, BA2 7AY, UK. a.t.a.jenkins@bath.ac.uk and School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.ORCID http://orcid.org/0000-0002-4095-2191
Adam C SedgwickDepartment of Chemistry, The University of Texas at Austin, 105 E 24th St A5300, Austin, Texas 78712-1224, USA.ORCID http://orcid.org/0000-0002-3132-2913
A Toby A JenkinsDepartment of Chemistry, University of Bath, BA2 7AY, UK. a.t.a.jenkins@bath.ac.uk.ORCID http://orcid.org/0000-0002-8981-3029
University of Bath · GBEast China University of Science and Technology · CNCardiff University · GBThe University of Texas at Austin · USUniversity of Kent · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alkaline phosphatase (ALP) is an important enzyme-based biomarker present in several bacterial species; however, it is currently undervalued as a strategy to detect pathogenic bacteria. Here, we explore our ALP-responsive colorimetric and fluorescent probe (TCF-ALP) for such applications. TCF-ALP displayed a colorimetric and fluorescence response towards Staphylococcus aureus (S. aureus), with a limit of detection of 3.7 × 106 CFU mL-1 after 24 h incubation. To our surprise, TCF-ALP proved selective towards Staphylococcus bacteria when compared with Enterococcus faecalis (E. faecalis), and Gram-negative P. aeruginosa and E. coli. Selectivity was also seen in clinically relevant S. aureus biofilms. Owing to the high prevalence and surface location of S. aureus in chronic wounds, TCF-ALP was subsequently encapsulated in polyvinyl alcohol (PVA)-based hydrogels as a proof-of-concept "smart" wound dressing. TCF-ALP hydrogels were capable of detecting S. aureus in planktonic and biofilm assays, and displayed a clear colour change from yellow to purple after 24 h incubation using ex vivo porcine skin models. Overall, TCF-ALP is a simple tool that requires no prior knowledge, training, or specialist equipment, and has the potential to overcome issues related to invasive swabbing and tissue biopsy methods. Thus, TCF-ALP could be used as a tool to monitor the early development of infection in a wound and allow for the rapid provision of appropriate treatment for Staphylococcal bacterial infections.

Indexed as

Alkaline PhosphataseStaphylococcus aureusAnimalsBacteriaBandagesBiofilmsEscherichia coliFluorescent DyesPseudomonas aeruginosaSwineAlkaline PhosphataseFluorescent Dyes

Identifiers

PMID34075906
PMCPMC8204156
OpenAlexW3162657751

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.