Band-pass filter-assisted ratiometric fluorescent nanoprobe composed of N-(2-aminoethyl-1,8-naphthalimide)-functionalized gold nanoclusters for the determination of alkaline phosphatase using digital image analysis.

Nana Cao, Jinjin Hou, Qihou Chen, Cheng Zhang, Jian Zhang, Yudie Sun, Qian Chen, Lifang He, Kui Zhang

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Article in Mikrochimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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9 authors.

Nana CaoSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China.

Jinjin HouSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China.

Qihou ChenSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China. chh123@ahut.edu.cn.

Cheng ZhangSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China.

Jian ZhangSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China. ahutjz@tech.ahut.edu.cn.

Yudie SunSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China.

Qian ChenSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China.

Lifang HeSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China.

Kui ZhangSchool of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243032, Anhui, China. zhangkui@mail.ustc.edu.cn.ORCID 0000-0002-5634-9952

Funding

National Natural Science Foundation of China 21976002, 22004003, 21675158 and 21507134

6 · The paper itself

Abstract

A smartphone-based dual-wavelength digital imaging platform containing red (539-695 nm) and blue (389-511 nm) band-pass filters was developed for point-of-care (POC) testing of alkaline phosphatase (ALP) activity. The platform was based on dual-emitting fluorescent nanohybrids (AuNC@NAN), the ratiometric probe, which had a fluorescence "on-off-on-off" response. The probe comprised red-emitting gold nanoclusters (AuNCs) acting as the signal report units and blue-emitting N-(2-aminoethyl-1,8-naphthalimide) (NAN) acting as an internal reference. The different responses of the ratiometric probes resulted in a continuous color-multiplexing change from pink-red to dark-purple upon exposure to ALP. The dual-wavelength digital imaging platform was employed to acquire images of AuNC or NAN fluorescence signals without the influence of background light. Unlike the classical one-time digital imaging mode, the accurate red (R) and blue (B) channel values of the generated images can help to directly judge or eliminate the disturbance from unavoidable interfering factors. The R/B values were successfully employed for determining the ALP activity at a range 2.0 to 35.0 mU·mL

Indexed as

Alkaline PhosphataseCell PhoneFluorescent DyesGoldHumansLimit of DetectionMetal NanoparticlesNaphthalimidesPoint-of-Care SystemsSpectrometry, FluorescenceAlkaline PhosphataseFluorescent DyesGoldNaphthalimidesFluorescent nanohybridsGold nanoclustersPoint-of-care testingSmartphone detectionVisualization detection

Identifiers

PMID34075479

DOI10.1007/s00604-021-04870-6

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