ArticleNature protocols2021
Spatial visualization of comprehensive brain neurotransmitter systems and neuroactive substances by selective in situ chemical derivatization mass spectrometry imaging.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Improving detection of low-abundant pharmaceuticals and neurotransmitters via quadrupole isolation in MALDI mass spectrometry imaging.Analytical and bioanalytical chemistry · 2026Article
- Chemical Derivatization Strategies for Expanding Small Biomolecule Analysis by MALDI-MS.Metabolites · 2026Review
- Altered Retinal Dopamine Homeostasis in Murine Models of Retinitis Pigmentosa.Journal of neurochemistry · 2026Article
- Anabolic androgenic steroids induce distinct monoaminergic and lipidomic alterations in the male rat brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- A head-to-tail dimeric J-aggregate probe for rapid and ratiometric imaging of mitochondrial 5-HTChemical science · 2026Article
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- Fast multimodal imaging combined with machine learning identifying taurine as a potential marker for breast cancer margin assessment.NPJ digital medicine · 2025Article
- Finding and filling the knowledge gaps in mechanisms of T cell-mediated TB immunity to inform vaccine design.Nature reviews. Immunology · 2025Review
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Quantitative Mass Spectrometry Imaging Protocols for Spatially Heterogeneous Samples.Analytical chemistry · 2025Article
- Preserving Neuronal Chemical Messengers: Heat Stabilization Versus Snap Freezing for Improved MALDI Mass Spectrometry Imaging of Brain Tissues.Journal of neurochemistry · 2025Article
- Met-ID: An Open-Source Software for Comprehensive Annotation of Multiple On-Tissue Chemical Modifications in MALDI-MSI.Analytical chemistry · 2025Article
- Protocols for Metabolomic Analyses to Assess Changes in Central Carbon Metabolism from Neural Tissue.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues.Analytical and bioanalytical chemistry · 2024Article
- Challenges in Spatial Metabolomics and Proteomics for Functional Tissue Unit and Single-Cell Resolution.Seminars in nephrology · 2024Review
- Morphological and molecular preservation through universal preparation of fresh-frozen tissue samples for multimodal imaging workflows.Nature protocols · 2024Review
- Early biomarkers in the presymptomatic phase of cognitive impairment: changes in the endocannabinoidome and serotonergic pathways in Alzheimer's-prone mice after mTBI.Acta neuropathologica communications · 2024Article
- Pharmacometabolomics and mass spectrometry imaging approach to reveal the neurochemical mechanisms ofJournal of pharmaceutical analysis · 2024Article
- Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level.ACS central science · 2024Review
- Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Molecule-specific techniques such as MALDI and desorption electrospray ionization mass spectrometry imaging enable direct and simultaneous mapping of biomolecules in tissue sections in a single experiment. However, neurotransmitter imaging in the complex environment of biological samples remains challenging. Our covalent charge-tagging approach using on-tissue chemical derivatization of primary and secondary amines and phenolic hydroxyls enables comprehensive mapping of neurotransmitter networks. Here, we present robust and easy-to-use chemical derivatization protocols that facilitate quantitative and simultaneous molecular imaging of complete neurotransmitter systems and drugs in diverse biological tissue sections with high lateral resolution. This is currently not possible with any other imaging technique. The protocol, using fluoromethylpyridinium and pyrylium reagents, describes all steps from tissue preparation (~1 h), chemical derivatization (1-2 h), data collection (timing depends on the number of samples and lateral resolution) and data analysis and interpretation. The specificity of the chemical reaction can also help users identify unknown chemical identities. Our protocol can reveal the cellular locations in which signaling molecules act and thus shed light on the complex responses that occur after the administration of drugs or during the course of a disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.