ArticleNPJ microgravity2021
3D cell culture using a clinostat reproduces microgravity-induced skin changes.
Article in NPJ microgravity, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Time-averaged simulated microgravity ameliorates tau-induced deficit in Drosophila melanogaster.NPJ microgravity · 2026Article
- Spherical Skin Model: Stratified Co-Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening.Advanced healthcare materials · 2026Article
- Dielectric double shell characterization of yeast cells exposed to simulated microgravity.NPJ microgravity · 2026Article
- Hardware-independent control for partial gravity simulation using a 2-DOF robotic device.Scientific reports · 2026Article
- Comparison of clinostat control strategies to achieve simulated microgravity with uniform gravity vector distribution.NPJ microgravity · 2026Article
- A biological and ethical assessment of whether humans could or should reproduce in space.NPJ microgravity · 2025Review
- Application of Microgravity Experiments in Plastic Surgery: A Literature Review.Plastic and reconstructive surgery. Global open · 2025Article
- The Proliferation of Chang Liver Cells After Simulated Microgravity Induction.Current issues in molecular biology · 2025Article
- Exploring the effects of simulated microgravity on esophageal cancer cells: insights into morphological, growth behavior, adhesion, and genetic damage.Journal of biological physics · 2024Article
- Effects of simulated microgravity on colorectal cancer organoids growth and drug response.Scientific reports · 2024Article
- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Fluid and Bubble Flow Detach Adherent Cancer Cells to Form Spheroids on a Random Positioning Machine.Cells · 2023Article
- Three-Dimensional Cell Cultures: The Bridge between In Vitro and In Vivo Models.International journal of molecular sciences · 2023Review
- The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on theCells · 2023Article
- 3D cell culture model: From ground experiment to microgravity study.Frontiers in bioengineering and biotechnology · 2023Review
- 3D microenvironment attenuates simulated microgravity-mediated changes in T cell transcriptome.Cellular and molecular life sciences : CMLS · 2022Article
- In Vitro Models of Bone Marrow Remodelling and Immune Dysfunction in Space: Present State and Future Directions.Biomedicines · 2022Review
- Spaceflight Stressors and Skin Health.Biomedicines · 2022Review
- Space Flight-Promoted Insulin Resistance as a Possible Disruptor of Wound Healing.Frontiers in bioengineering and biotechnology · 2022Review
- Fibroblast Differentiation and Matrix Remodeling Impaired under Simulated Microgravity in 3D Cell Culture Model.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exposure to microgravity affects human physiology in various ways, and astronauts frequently report skin-related problems. Skin rash and irritation are frequent complaints during space missions, and skin thinning has also been reported after returning to Earth. However, spaceflight missions for studying the physiological changes in microgravity are impractical. Thus, we used a previously developed 3D clinostat to simulate a microgravity environment and investigate whether physiological changes of the skin can be reproduced in a 3D in vitro setting. Our results showed that under time-averaged simulated microgravity (taSMG), the thickness of the endothelial cell arrangement increased by up to 59.75%, indicating skin irritation due to vasodilation, and that the diameter of keratinocytes and fibroblast co-cultured spheroids decreased by 6.66%, representing skin thinning. The α1 chain of type I collagen was upregulated, while the connective tissue growth factor was downregulated under taSMG. Cytokeratin-10 expression was significantly increased in the taSMG environment. The clinostat-based 3D culture system can reproduce physiological changes in the skin similar to those under microgravity, providing insight for understanding the effects of microgravity on human health before space exploration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.