ArticleProceedings of the National Academy of Sciences of the United States of America2021
Single-molecule calorimeter and free energy landscape.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- From biomolecular condensates to functional nanomaterials: LLPS-inspired frameworks for nanoscale hydrogels and adaptive materials.Journal of nanobiotechnology · 2026Review
- Large field-of-view plasmonic scattering imaging and sensing of nanoparticles with isotropic point-spread-function.Nature communications · 2025Article
- Single molecule-driven nanomotors reveal the dynamic-disordered chemomechanical transduction of active enzymes.Science advances · 2025Article
- Dielectric Surface-Based Biosensors for Enhanced Detection of Biomolecular Interactions: Advances and Applications.Biosensors · 2024Review
- Label-Free Optical Imaging of Nanoscale Single Entities.ACS sensors · 2024Review
- Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution.Biosensors · 2024Review
- Plasmonic Scattering Microscopy for Label-Free Imaging of Molecular Binding Kinetics: From Single Molecules to Single Cells.Chemistry methods : new approaches to solving problems in chemistry · 2023Article
- A generalizable nanopore sensor for highly specific protein detection at single-molecule precision.Nature communications · 2023Article
- Rapid Regulation of Local Temperature and Transient Receptor Potential Vanilloid 1 Ion Channels with Wide-Field Plasmonic Thermal Microscopy.Analytical chemistry · 2022Article
- In Situ Analysis of Membrane-Protein Binding Kinetics and Cell-Surface Adhesion Using Plasmonic Scattering Microscopy.Angewandte Chemie (International ed. in English) · 2022Article
- Evanescent scattering imaging of single protein binding kinetics and DNA conformation changes.Nature communications · 2022Article
- Label-Free Imaging of Nanoscale Displacements and Free-Energy Profiles of Focal Adhesions with Plasmonic Scattering Microscopy.ACS sensors · 2021Article
- Thermodynamic profiling of single molecules.Nature methods · 2021Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The precise measurement of thermodynamic and kinetic properties for biomolecules provides the detailed information for a multitude of applications in biochemistry, biosensing, and health care. However, sensitivity in characterizing the thermodynamic binding affinity down to a single molecule, such as the Gibbs free energy ([Formula: see text]), enthalpy ([Formula: see text]), and entropy ([Formula: see text]), has not materialized. Here, we develop a nanoparticle-based technique to probe the energetic contributions of single-molecule binding events, which introduces a focused laser of optical tweezer to an optical path of plasmonic imaging to accumulate and monitor the transient local heating. This single-molecule calorimeter uncovers the complex nature of molecular interactions and binding characterizations, which can be employed to identify the thermodynamic equilibrium state and determine the energetic components and complete thermodynamic profile of the free energy landscape. This sensing platform promises a breakthrough in measuring thermal effect at the single-molecule level and provides a thorough description of biomolecular specific interactions.
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