Evidence map›Paper›PMID 34073778›Full record

ArticleCells2021

Genome Editing to Generate Sake Yeast Strains with Eight Mutations That Confer Excellent Brewing Characteristics.

Tomoya Chadani, Shinsuke Ohnuki, Atsuko Isogai, Tetsuya Goshima, Mao Kashima, Farzan Ghanegolmohammadi, Tomoyuki Nishi, Dai Hirata, Daisuke Watanabe, Katsuhiko Kitamoto and 2 more

Abstract read
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. The Use of Non-Conventional Yeast in Sake Production.Molecules (Basel, Switzerland) · 2025
    Article
  2. Article
  3. Article
  4. Article
  5. Defining Functions of Mannoproteins inJournal of fungi (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Tomoya ChadaniDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
Shinsuke OhnukiDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
Atsuko IsogaiNational Research Institute of Brewing, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Tetsuya GoshimaNational Research Institute of Brewing, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Mao KashimaDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
Farzan GhanegolmohammadiDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.ORCID 0000-0001-5217-0878
Tomoyuki NishiSake Research Center, Asahi Sake Brewing Co. Ltd., Nagaoka, Niigata 949-5494, Japan.
Dai HirataSake Research Center, Asahi Sake Brewing Co. Ltd., Nagaoka, Niigata 949-5494, Japan.
Daisuke WatanabeGraduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Katsuhiko KitamotoDepartment of Pharmaceutical and Medical Business Sciences, Nihon Pharmaceutical University, Bunkyo-ku, Tokyo 113-0034, Japan.
Takeshi AkaoNational Research Institute of Brewing, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Yoshikazu OhyaDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.ORCID 0000-0003-0837-1239

Funding

JSPS KAKENHI 19H03205
6 · The paper itself

Abstract

Sake yeast is mostly diploid, so the introduction of recessive mutations to improve brewing characteristics requires considerable effort. To construct sake yeast with multiple excellent brewing characteristics, we used an evidence-based approach that exploits genome editing technology. Our breeding targeted the

Indexed as

Gene EditingAlcoholic BeveragesDiploidyFermentationMutationOdorantsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSaccharomyces cerevisiae ProteinsCAR1CRISPR/Cas9FAS2genome editingMDE1sake yeast

Identifiers

PMID34073778
PMCPMC8225151

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.