ArticleLife (Basel, Switzerland)2021
A Novel Assay for Phosphoserine Phosphatase Exploiting Serine Acetyltransferase as the Coupling Enzyme.
Article in Life (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Phosphoserine as an Alternative Energy Source forACS synthetic biology · 2026Article
- Post-translational regulation of human D-3-phosphoglycerate dehydrogenase in Alzheimer's disease.Protein science : a publication of the Protein Society · 2026Article
- Selenium nanoparticles enhance innate immunity in aphid-stressed oat plants by modulating photosynthetic pigments, sugar, nitrogen and fatty acid metabolism, and antioxidant defense.BMC plant biology · 2026Article
- A phosphoserine phosphatase variant present in the brain of Alzheimer's disease patients favors nuclear mistargeting.The FEBS journal · 2025Article
- First-in-class inhibitors of SbnA reduce siderophore production in Staphylococcus aureus.The FEBS journal · 2025Article
- Human citrate synthase kinetic simulation to fit rapid, direct, and thiol probe coupled kinetic data.Biochemistry and biophysics reports · 2025Article
- Exploring the benefits of AMF colonization for improving wheat growth, physiology and metabolism, and antimicrobial activity under biotic stress from aphid infection.BMC plant biology · 2025Article
- Catalysts for sulfur: understanding the intricacies of enzymes orchestrating plant sulfur anabolism.Planta · 2024Review
- The unique catalytic properties of PSAT1 mediate metabolic adaptation to glutamine blockade.Nature metabolism · 2024Article
- L-serine deficiency: on the properties of the Asn133Ser variant of human phosphoserine phosphatase.Scientific reports · 2024Article
- Phosphoserine Aminotransferase Pathogenetic Variants in Serine Deficiency Disorders: A Functional Characterization.Biomolecules · 2023Article
- L-serine biosynthesis in the human central nervous system: Structure and function of phosphoserine aminotransferase.Protein science : a publication of the Protein Society · 2023Article
- Effects of Date Palm Waste Compost Application on Root Proteome Changes of Barley (Plants (Basel, Switzerland) · 2023Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Phosphoserine phosphatase (PSP) catalyzes the final step of de novo L-serine biosynthesis-the hydrolysis of phosphoserine to serine and inorganic phosphate-in humans, bacteria, and plants. In published works, the reaction is typically monitored through the discontinuous malachite green phosphate assay or, more rarely, through a continuous assay that couples phosphate release to the phosphorolysis of a chromogenic nucleoside by the enzyme purine nucleoside phosphorylase (PNP). These assays suffer from numerous drawbacks, and both rely on the detection of phosphate. We describe a new continuous assay that monitors the release of serine by exploiting bacterial serine acetyltransferase (SAT) as a reporter enzyme. SAT acetylates serine, consuming acetyl-CoA and releasing CoA-SH. CoA-SH spontaneously reacts with Ellman's reagent to produce a chromophore that absorbs light at 412 nm. The catalytic parameters estimated through the SAT-coupled assay are fully consistent with those obtained with the published methods, but the new assay exhibits several advantages. Particularly, it depletes L-serine, thus allowing more prolonged linearity in the kinetics. Moreover, as the SAT-coupled assay does not rely on phosphate detection, it can be used to investigate the inhibitory effect of phosphate on PSP.
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