Evidence map›Paper›PMID 34071819›Full record

ArticleViruses2021

The HIV 5' Gag Region Displays a Specific Nucleotide Bias Regulating Viral Splicing and Infectivity.

Bastian Grewe, Carolin Vogt, Theresa Horstkötter, Bettina Tippler, Han Xiao, Bianca Müller, Klaus Überla, Ralf Wagner, Benedikt Asbach, Jens Bohne

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Bastian GreweDepartment of Molecular and Medical Virology, Ruhr-University, 44801 Bochum, Germany.
Carolin VogtInstitute of Virology, Hannover Medical School, 30625 Hannover, Germany.
Theresa HorstkötterInstitute of Virology, Hannover Medical School, 30625 Hannover, Germany.
Bettina TipplerDepartment of Molecular and Medical Virology, Ruhr-University, 44801 Bochum, Germany.
Han XiaoDepartment of Molecular and Medical Virology, Ruhr-University, 44801 Bochum, Germany.
Bianca MüllerDepartment of Molecular and Medical Virology, Ruhr-University, 44801 Bochum, Germany.
Klaus ÜberlaDepartment of Molecular and Medical Virology, Ruhr-University, 44801 Bochum, Germany.ORCID 0000-0001-7822-3835
Ralf WagnerInstitute of Medical Microbiology and Hygiene, University Regensburg, 93053 Regensburg, Germany.
Benedikt AsbachInstitute of Medical Microbiology and Hygiene, University Regensburg, 93053 Regensburg, Germany.ORCID 0000-0003-1056-8591
Jens BohneInstitute of Virology, Hannover Medical School, 30625 Hannover, Germany.
Ruhr University Bochum · DEMedizinische Hochschule Hannover · DEInstitute of Medical Microbiology and Hygiene · DEUniversity Hospital Regensburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alternative splicing and the expression of intron-containing mRNAs is one hallmark of HIV gene expression. To facilitate the otherwise hampered nuclear export of non-fully processed mRNAs, HIV encodes the Rev protein, which recognizes its intronic response element and fuels the HIV RNAs into the CRM-1-dependent nuclear protein export pathway. Both alternative splicing and Rev-dependency are regulated by the primary HIV RNA sequence. Here, we show that these processes are extremely sensitive to sequence alterations in the 5'coding region of the HIV genomic RNA. Increasing the GC content by insertion of either GFP or silent mutations activates a cryptic splice donor site in

Indexed as

RNA SplicingAlternative Splicinggag Gene Products, Human Immunodeficiency VirusHEK293 CellsHIV-1HIV InfectionsHumansRNA, MessengerRNA Splice SitesRNA, ViralVirus Replicationgag Gene Products, Human Immunodeficiency VirusRNA, MessengerRNA Splice SitesRNA, Viralalternative splicingHIV gene expressionmRNP codeU1 snRNP

Identifiers

PMID34071819
PMCPMC8227319
OpenAlexW3165469971

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.