Evidence map›Paper›PMID 34070520›Full record

ArticleMedical sciences (Basel, Switzerland)2021

Reduced Serum Circulation of Cell-Free DNA Following Chemotherapy in Breast Cancer Patients.

Evelyn Adusei, John Ahenkorah, Nii Armah Adu-Aryee, Kevin Kofi Adutwum-Ofosu, Emmanuel Ayitey Tagoe, Nii Koney-Kwaku Koney, Emmanuel Nkansah, Nii Ayite Aryee, Richard Michael Blay, Bismarck Afedo Hottor and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical sciences (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Evelyn AduseiDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.
John AhenkorahDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.ORCID 0000-0002-0617-5210
Nii Armah Adu-AryeeDepartment of Surgery, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.
Kevin Kofi Adutwum-OfosuDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.ORCID 0000-0002-3731-3399
Emmanuel Ayitey TagoeDepartment of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra P.O. Box KB 143, Ghana.ORCID 0000-0001-7179-1872
Nii Koney-Kwaku KoneyDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.
Emmanuel NkansahDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.
Nii Ayite AryeeDepartment of Medical Biochemistry, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.
Richard Michael BlayDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.ORCID 0000-0002-5356-4323
Bismarck Afedo HottorDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.ORCID 0000-0002-0286-473X
Joe-Nat Clegg-LampteyDepartment of Surgery, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.ORCID 0000-0003-0497-071X
Benjamin Arko-BohamDepartment of Anatomy, University of Ghana Medical School, University of Ghana, Accra P.O. Box GP 4236, Ghana.ORCID 0000-0001-6429-2186

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Breast cancer is the most common malignancy in women, with alarming mortalities. Neoadjuvant treatments employ chemotherapy to shrink tumours to a well-defined size for a better surgical outcome. The current means of assessing effectiveness of chemotherapy management are imprecise. We previously showed that breast cancer patients have higher serum circulating cell-free DNA concentrations. cfDNA is degraded cellular DNA fragments released into the bloodstream. We further report on the utility of cfDNA in assessing the response to chemotherapy and its potential as a monitoring biomarker. A total of 32 newly diagnosed and treatment-naive female breast cancer patients and 32 healthy females as controls were included. Anthropometric, demographic and clinicopathological information of participants were recorded. Each participant donated 5 mL of venous blood from which sera were separated. Blood sampling was carried out before the commencement of chemotherapy (timepoint 1) and after the third cycle of chemotherapy (timepoint 2). qPCR was performed on the sera to quantify ALU 115 and 247 levels, and DNA integrity (ALU247/ALU115) was determined. ALU 115 and 247 levels were elevated in cancer patients but were significantly decreased after the third cycle of chemotherapy (T2) compared to T1. DNA integrity increased after the third cycle. Serum cfDNA may provide a relatively inexpensive and minimally invasive procedure to evaluate the response to chemotherapy in breast cancer.

Indexed as

Breast NeoplasmsCell-Free Nucleic AcidsBiomarkers, TumorDNAFemaleHumansNeoadjuvant TherapyBiomarkers, TumorCell-Free Nucleic AcidsDNAbiomarkerbreast cancercfDNAchemotherapycirculating cell-free DNA

Identifiers

PMID34070520
PMCPMC8163010

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.