Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1

Rebecca San Gil, Benjamin E Clarke, Heath Ecroyd, Bernadett Kalmar, Linda Greensmith

Open access · goldAbstract readComparative Study

In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

0.2field-weighted citation impact, top 48% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

Use of Proteomics to Explore Biomarkers of Amyotrophic Lateral Sclerosis (ALS): Proof of Principle from Humanized SOD1 Mouse to Human ALS.ACS pharmacology & translational science · 2025
Review
Expression of LIM domain-binding 3 (LDB3), a striated muscle Z-band alternatively spliced PDZ-motif protein in the nervous system.Scientific reports · 2023
Article
Perturbations of the Proteome and of Secreted Metabolites in Primary Astrocytes from the hSOD1(G93A) ALS Mouse Model.International journal of molecular sciences · 2021
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Rebecca San GilMolecular Horizons and School of Chemistry and Molecular Bioscience, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2519, Australia.ORCID 0000-0001-5725-5512

Benjamin E ClarkeDepartment of Neuromuscular Disease, UCL Queen Square Institute of Neurology, London WC1N 3BG, UK.ORCID 0000-0001-5775-352X

Heath EcroydMolecular Horizons and School of Chemistry and Molecular Bioscience, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2519, Australia.ORCID 0000-0001-7574-0044

Bernadett KalmarDepartment of Neuromuscular Disease, UCL Queen Square Institute of Neurology, London WC1N 3BG, UK.

Linda GreensmithDepartment of Neuromuscular Disease, UCL Queen Square Institute of Neurology, London WC1N 3BG, UK.

National Hospital for Neurology and Neurosurgery · GBThe Francis Crick Institute · GBThe University of Queensland · AUUniversity of Wollongong · AU

Funding

UK Medical Research Council Centre for Neuromuscular Diseases G0601943

6 · The paper itself

Abstract

Heterogeneity of glia in different CNS regions may contribute to the selective vulnerability of neuronal populations in neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS). Here, we explored regional variations in the expression of heat shock protein 25 in glia under conditions of acute and chronic stress. Hsp27 (Hsp27; murine orthologue: Hsp25) fulfils a number of cytoprotective functions and may therefore be a possible therapeutic target in ALS. We identified a subpopulation of astrocytes in primary murine mixed glial cultures that expressed Hsp25. Under basal conditions, the proportion of Hsp25-positive astrocytes was twice as high in spinal cord cultures than in cortical cultures. To explore the physiological role of the elevated Hsp25 expression in spinal cord astrocytes, we exposed cortical and spinal cord glia to acute stress, using heat stress and pro-inflammatory stimuli. Surprisingly, we observed no stress-induced increase in Hsp25 expression in either cortical or spinal cord astrocytes. Similarly, exposure to endogenous stress, as modelled in glial cultures from SOD1

Indexed as

AnimalsAstrocytesCells, CulturedCerebral CortexFemaleGene Expression RegulationGliosisHeat-Shock ProteinsHeat-Shock ResponseHumansLipopolysaccharidesMaleMiceMice, Inbred C57BLMice, TransgenicMolecular ChaperonesHeat-Shock ProteinsHsbp1 protein, mouseLipopolysaccharidesMolecular ChaperonesSOD1 protein, humanSuperoxide Dismutase-1Tumor Necrosis Factor-alphaamyotrophic lateral sclerosisastrocytesgliaheat shock protein 27heat shock responsemotor neuron disease

Identifiers

PMID34069691

DOI10.3390/cells10051257

PMCPMC8160835

OpenAlexW3163123026

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.