ArticleBiomolecules2021
The Combination of Paraformaldehyde and Glutaraldehyde Is a Potential Fixative for Mitochondria.
Article in Biomolecules, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
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- Protocol for visualizing microglial lysosomal content by immunohistochemistry in the rodent brain.STAR protocols · 2026Article
- DNA2 acts as a brake on β cell insulin hypersecretion and diet-induced metabolic dysfunction.Frontiers in cell and developmental biology · 2026Article
- Automated morphological classification and quantification of cerebrospinal fluid extracellular vesiclesNanoscale advances · 2025Article
- Stain-free artificial intelligence-assisted light microscopy for the identification of leukocyte morphology change in presence of bacteria.Frontiers in bioinformatics · 2025Article
- Approaching maximum resolution in structured illumination microscopy via accurate noise modeling.Npj imaging · 2025Article
- Hypoxic preconditioning increases mitochondrial respiration and HFrontiers in molecular neuroscience · 2025Article
- Chemical Landscape of Adipocytes Derived from 3T3-L1 Cells Investigated by Fourier Transform Infrared and Raman Spectroscopies.International journal of molecular sciences · 2024Article
- Approaching Maximum Resolution in Structured Illumination Microscopy via Accurate Noise Modeling.bioRxiv : the preprint server for biology · 2024Article
- Article
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- Sea buckthorn, its bioactive constituents, and mechanism of action: potential application in female reproduction.Frontiers in endocrinology · 2023Review
- LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes' Shift.International journal of molecular sciences · 2022Article
- Glutamate Receptors Mediate Changes to Dendritic Mitochondria in Neurons Grown on Stiff Substrates.Annals of biomedical engineering · 2022Article
- Neuronal mitochondrial morphology is significantly affected by both fixative and oxygen level during perfusion.Frontiers in molecular neuroscience · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Mitochondria are highly dynamic organelles, constantly undergoing shape changes, which are controlled by mitochondrial movement, fusion, and fission. Mitochondria play a pivotal role in various cellular processes under physiological and pathological conditions, including metabolism, superoxide generation, calcium homeostasis, and apoptosis. Abnormal mitochondrial morphology and mitochondrial protein expression are always closely related to the health status of cells. Analysis of mitochondrial morphology and mitochondrial protein expression in situ is widely used to reflect the abnormality of cell function in the chemical fixed sample. Paraformaldehyde (PFA), the most commonly used fixative in cellular immunostaining, still has disadvantages, including loss of antigenicity and disruption of morphology during fixation. We tested the effect of ethanol (ETHO), PFA, and glutaraldehyde (GA) fixation on cellular mitochondria. The results showed that 3% PFA and 1.5% GA (PFA-GA) combination reserved mitochondrial morphology better than them alone in situ in cells. Mitochondrial network and protein antigenicity were well maintained, indicated by preserved MitoTracker and mitochondrial immunostaining after PFA-GA fixation. Our results suggest that the PFA-GA combination is a valuable fixative for the study of mitochondria in situ.
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Registered trials
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