Evidence map›Paper›PMID 34068362›Full record

ArticleGenes2021

Identification and Validation of Reference Genes for Gene Expression Analysis in

Zhongyi Yang, Rui Zhang, Zhichun Zhou

Open access · goldAbstract read
In one paragraph

Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 36 citations in OpenAlex.

  1. Selection and Validation of qRT-PCR Reference Genes inCurrent issues in molecular biology · 2026
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  8. Article
  9. Reference Genes Selection and Validation forInternational journal of molecular sciences · 2024
    Article
  10. Article
  11. Article
  12. Genome-Wide Identification and Expression Profiling of Potato (International journal of molecular sciences · 2024
    Article
  13. Article
  14. Article
  15. Selection of Reference Genes inPlants (Basel, Switzerland) · 2023
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Zhongyi YangResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.ORCID 0000-0001-6516-918X
Rui ZhangResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Zhichun ZhouResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Chinese Academy of Forestry · CNNanjing Forestry University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene expression studies, but its accuracy depends on the expression stability of reference genes.

Indexed as

Gene ExpressionGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantGenetic TechniquesReal-Time Polymerase Chain ReactionReference StandardsSoftwareTheaceaereal-time quantitative PCRreference geneSchima superbastability evaluationtissues

Identifiers

PMID34068362
PMCPMC8153319
OpenAlexW3162525847

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.