A Label-Free Fluorometric Glutathione Assay Based on a Conformational Switch of G-quadruplex.

Xi Zhou, Doudou Zhang, Ying Yan, Hailun He, Yukui Zhou, Changbei Ma

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Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.1field-weighted citation impact, top 57% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Xi ZhouSchool of Life Sciences, Central South University, Changsha 410013, China.

Doudou ZhangSchool of Life Sciences, Central South University, Changsha 410013, China.

Ying YanSchool of Life Sciences, Central South University, Changsha 410013, China.

Hailun HeSchool of Life Sciences, Central South University, Changsha 410013, China.

Yukui ZhouSchool of Life Sciences, Central South University, Changsha 410013, China.

Changbei MaSchool of Life Sciences, Central South University, Changsha 410013, China.

Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this paper, a label-free fluorescent method for glutathione (GSH) detection based on a thioflavin T/G-quadruplex conformational switch is developed. The sensing assay is fabricated depending on the virtue of mercury ions to form a thymine-thymine mismatch, which collapses the distance between two ssDNA and directs the guanine-rich part to form an intra-strand asymmetric split G-quadruplex. The newly formed G-quadruplex efficiently reacts with thioflavin T and enhances the fluorescent intensity. In the presence of GSH, Hg

Indexed as

FluorometryG-QuadruplexesNucleic Acid ConformationStaining and LabelingBiological AssayFeasibility StudiesGlutathioneReproducibility of ResultsSpectrometry, FluorescenceGlutathionefluorescence assayglutathioneG-quadruplexmercury ionsthioflavin T

Identifiers

PMID34066991

DOI10.3390/molecules26092743

PMCPMC8124632

OpenAlexW3158346653

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.