Evidence map›Paper›PMID 34066245›Full record

ArticleMolecules (Basel, Switzerland)2021

Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization.

Kristina Westerlund, Anders Myrhammar, Hanna Tano, Maxime Gestin, Amelie Eriksson Karlström

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Expression and Characterization of Intein-Cyclized Trimer ofInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Kristina WesterlundDepartment of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, SE-10691 Stockholm, Sweden.ORCID 0000-0003-1610-7614
Anders MyrhammarDepartment of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, SE-10691 Stockholm, Sweden.
Hanna TanoDepartment of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, SE-10691 Stockholm, Sweden.
Maxime GestinDepartment of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, SE-10691 Stockholm, Sweden.ORCID 0000-0002-4164-166X
Amelie Eriksson KarlströmDepartment of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, SE-10691 Stockholm, Sweden.ORCID 0000-0002-0695-5188
AlbaNova · SE

Funding

Swedish Research Council 2016-05207
6 · The paper itself

Abstract

Natural backbone-cyclized proteins have an increased thermostability and resistance towards proteases, characteristics that have sparked interest in head-to-tail cyclization as a method to stability-enhance proteins used in diagnostics and therapeutic applications, for example. In this proof-of principle study, we have produced and investigated a head-to-tail cyclized and HER2-specific Z

Indexed as

BiocatalysisProtein MultimerizationAminoacyltransferasesBacterial ProteinsBreast NeoplasmsCircular DichroismCyclizationCysteine EndopeptidasesErb-b2 Receptor Tyrosine KinasesFemaleHumansKineticsMCF-7 CellsMicroscopy, FluorescencePeptide HydrolasesProtein Bindingaffibody (ZHER2-342)2AminoacyltransferasesBacterial ProteinsCysteine EndopeptidasesERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesPeptide HydrolasesRecombinant Fusion Proteinssortase AAffibodycyclizationproteolytic stabilitysortase Athermal stability

Identifiers

PMID34066245
PMCPMC8150554
OpenAlexW3091911488

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.