Evidence map›Paper›PMID 34064959›Full record

ArticleViruses2021

A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants.

Eva C Thuenemann, Matthew J Byrne, Hadrien Peyret, Keith Saunders, Roger Castells-Graells, Inmaculada Ferriol, Mattia Santoni, John F C Steele, Neil A Ranson, Linda Avesani and 2 more

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 24 citations in OpenAlex.

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  11. The Use of a Replicating Virus Vector ForFrontiers in bioengineering and biotechnology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 4 countries.

Eva C ThuenemannJohn Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK.ORCID 0000-0002-9083-2593
Matthew J ByrneAstbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Hadrien PeyretJohn Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK.ORCID 0000-0002-7808-5089
Keith SaundersJohn Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK.ORCID 0000-0002-5606-9627
Roger Castells-GraellsJohn Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK.ORCID 0000-0002-3985-6194
Inmaculada FerriolCentre for Research in Agricultural Genomics (CRAG, CSIC-IRTA-UAB-UB), 08193 Cerdanyola del Vallès, Spain.ORCID 0000-0003-0111-7537
Mattia SantoniDiamante srl. Strada Le Grazie, 15, 37134 Verona, Italy.ORCID 0000-0001-5694-7605
John F C SteeleJohn Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK.ORCID 0000-0002-4388-4956
Neil A RansonAstbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.ORCID 0000-0002-3640-5275
Linda AvesaniDepartment of Biotechnology, University of Verona, Strada Le Grazie, 15, 37134 Verona, Italy.ORCID 0000-0003-4007-7013
Juan Jose Lopez-MoyaCentre for Research in Agricultural Genomics (CRAG, CSIC-IRTA-UAB-UB), 08193 Cerdanyola del Vallès, Spain.ORCID 0000-0002-3176-9651
George P LomonossoffJohn Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK.ORCID 0000-0003-0700-906X
John Innes Centre · GBConsejo Superior de Investigaciones Científicas · ESUniversity of Leeds · GBUniversity of Verona · IT

Funding

Biotechnology and Biological Sciences Research Council BB/R001669/1Biotechnology and Biological Sciences Research Council BB/T004703/1
6 · The paper itself

Abstract

The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-

Indexed as

Transduction, GeneticVirus ReplicationCapsidGenetic VectorsNicotianaPlantsPlant Virusesfilamentoushigh-resolution cryo-EMoverexpressionpEAQ-HTpEffPotexvirusPotyviridaerod-shapedvirus-like particle

Identifiers

PMID34064959
PMCPMC8150850
OpenAlexW3161426717

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.