Evidence map›Paper›PMID 34064871›Full record

ArticleBiology2021

Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases.

Priyakshi Kalita-de Croft, Haarika Chittoory, Tam H Nguyen, Jodi M Saunus, Woo Gyeong Kim, Amy E McCart Reed, Malcolm Lim, Xavier M De Luca, Kaltin Ferguson, Colleen Niland and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

  1. Ionizing Radiation Enhances Prognostically Significant Cellular Immunity Programs in the Brain Metastasis Microenvironment.Clinical cancer research : an official journal of the American Association for Cancer Research · 2026
    Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Immune related biomarkers for cancer metastasis to the brain.Experimental hematology & oncology · 2022
    Review
  8. Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Priyakshi Kalita-de CroftUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.ORCID 0000-0001-8877-7655
Haarika ChittooryUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.
Tam H NguyenQIMR Berghofer Medical Research Institute, Herston, QLD 4029, Australia.
Jodi M SaunusUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.ORCID 0000-0002-4604-4709
Woo Gyeong KimUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.ORCID 0000-0003-1694-6602
Amy E McCart ReedUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.ORCID 0000-0001-5387-2791
Malcolm LimUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.
Xavier M De LucaUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.
Kaltin FergusonUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.
Colleen NilandUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.
Roberta MazzieriDiamantina Institute, Translational, Research Institute, The University of Queensland Diamantina Institute, Brisbane, QLD 4102, Australia.
Riccardo DolcettiDiamantina Institute, Translational, Research Institute, The University of Queensland Diamantina Institute, Brisbane, QLD 4102, Australia.ORCID 0000-0003-1625-9853
Peter T SimpsonUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.ORCID 0000-0002-4816-8289
Sunil R LakhaniUQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Herston, QLD 4029, Australia.ORCID 0000-0003-4067-2760
The University of Queensland · AUTranslational Research Institute · AUQIMR Berghofer Medical Research Institute · AU

Funding

National Health and Medical Research Council APP1017028
6 · The paper itself

Abstract

The heterogeneity of tumor infiltrating lymphocytes (TILs) is not well characterized in brain metastasis. To address this, we performed a targeted analysis of immune-cell subsets in brain metastasis tissues to test immunosuppressive routes involved in brain metastasis. We performed multiplex immunofluorescence (mIF), using commercially available validated antibodies on formalin-fixed paraffin embedded whole sections. We quantitated the subsets of immune-cells utilizing a targeted panel of proteins including PanCK, CD8, CD4, VISTA and IBA-1, and analyzed an average of 15,000 cells per sample. Classifying tumors as either high (>30%) or low (<30%) TILs, we found that increased TILs density correlated with survival. Phenotyping these TILs we found tumors with low TILs had significantly higher expression of the immune-checkpoint molecule VISTA in tumor cells (

Indexed as

brain metastasisimmune checkpointT-cellsTILstumor microenvironment

Identifiers

PMID34064871
PMCPMC8150725
OpenAlexW3129269493

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.