Polyoxazolines with a Vicinally Double-Bioactivated Terminus for Biomacromolecular Affinity Assessment.

Florian Pinzner, Thorsten Keller, Jürgen Mut, Julian Bechold, Jürgen Seibel, Jürgen Groll

Open access · goldAbstract read

In one paragraph

Article in Sensors (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.4field-weighted citation impact, top 39% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Florian PinznerDepartment of Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.

Thorsten KellerDepartment of Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.ORCID 0000-0002-2897-5761

Jürgen MutInstitute of Organic Chemistry, University of Würzburg, Am Hubland, 97070 Würzburg, Germany.

Julian BecholdInstitute of Organic Chemistry, University of Würzburg, Am Hubland, 97070 Würzburg, Germany.

Jürgen SeibelInstitute of Organic Chemistry, University of Würzburg, Am Hubland, 97070 Würzburg, Germany.

Jürgen GrollDepartment of Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.ORCID 0000-0003-3167-8466

University of Würzburg · DE

Funding

Deutsche Forschungsgemeinschaft 326998133

6 · The paper itself

Abstract

Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments.

Indexed as

GoldSurface Plasmon ResonanceConcanavalin ALectinsMannoseOxazolesConcanavalin AGoldLectinsMannoseOxazolesfunctionalizationlectinpolyoxazolines

Identifiers

PMID34062922

DOI10.3390/s21093153

PMCPMC8125408

OpenAlexW3159110201

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.