ArticleMolecular plant2021
Reconstruction of lateral root formation through single-cell RNA sequencing reveals order of tissue initiation.
Article in Molecular plant, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Gene Expression Analysis from Specific Root Cell Types.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Lateral Root Development: Molecular Mechanisms and Adaptive Regulation.Plant, cell & environment · 2026Review
- Structural Variation and Its Roles in Plant Genomes.Plants (Basel, Switzerland) · 2026Review
- Auxin promotes robust founder cell specification during Arabidopsis lateral root initiation.Genetics · 2026Article
- Single-nucleus sequencing and spatial metabolomics analysis reveal the regulatory mechanism of ginkgolic acid biosynthesis in the episperm ofHorticulture research · 2026Article
- Advances in single-cell and spatial omics for studying symbiotic nitrogen fixation: comparative cellular and evolutionary perspectives.Genome biology · 2026Review
- Prior-guided factorization for reliable imputation of scRNA-seq data.PLoS computational biology · 2026Article
- Modelling the short-term response to nitrogen that coordinates events in lateral root initiation.Quantitative plant biology · 2026Article
- Root growth promotion bybioRxiv : the preprint server for biology · 2025Article
- Single-cell views of fate reprogramming in de novo organogenesis.Journal of plant research · 2025Review
- Integrating 3D imaging, GWAS, and single-cell transcriptome approaches to elucidate root system architecture in Populus.Plant physiology · 2025Article
- Temporal regulation of gene expression during auxin-triggered crown root formation in barley: an integrated approach.Plant & cell physiology · 2025Article
- Strigolactone insensitivity affects differential shoot and root transcriptome in barley.Journal of applied genetics · 2025Article
- Transcriptional landscape of sweetpotato root tip development at the single-cell level.BMC plant biology · 2024Article
- Shaping root architecture: towards understanding the mechanisms involved in lateral root development.Biology direct · 2024Review
- What Is a Plant Cell Type in the Age of Single-Cell Biology? It's Complicated.Annual review of cell and developmental biology · 2024Review
- Systematic expression analysis of cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 protein (CAP) superfamily inPlant direct · 2024Article
- Single-Cell Transcriptomics Applied in Plants.Cells · 2024Review
- Single-cell network analysis reveals gene expression programs for Arabidopsis root development and metabolism.Plant communications · 2024Article
- A single-cell transcriptome atlas reveals the trajectory of early cell fate transition during callus induction in Arabidopsis.Plant communications · 2024Article
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Authors and funding
8 authors.
Funding
Abstract
Postembryonic organogenesis is critical for plant development. Underground, lateral roots (LRs) form the bulk of mature root systems, yet the ontogeny of the LR primordium (LRP) is not clear. In this study, we performed the single-cell RNA sequencing through the first four stages of LR formation in Arabidopsis. Our analysis led to a model in which a single group of precursor cells, with a cell identity different from their pericycle origins, rapidly reprograms and splits into a mixed ground tissue/stem cell niche fate and a vascular precursor fate. The ground tissue and stem cell niche fates soon separate and a subset of more specialized vascular cells form sucrose transporting phloem cells that appear to connect to the primary root. We did not detect cells resembling epidermis or root cap, suggesting that outer tissues may form later, preceding LR emergence. At this stage, some remaining initial precursor cells form the primordium flanks, while the rest create a reservoir of pluripotent cells that are able to replace the LR if damaged. Laser ablation of the central and lateral LRP regions showed that remaining cells restart the sequence of tissue initiation to form a LR. Collectively, our study reveals an ontological hierarchy for LR formation with an early and sequential split of main root tissues and stem cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.