Evidence map›Paper›PMID 34061306›Full record

ArticleApplied biochemistry and biotechnology2021

Function Enhancement of a Metabolic Module via Endogenous Promoter Replacement for Pseudomonas sp. JY-Q to Degrade Nicotine in Tobacco Waste Treatment.

Jun Li, Fengmei Yi, Guoqing Chen, Fanda Pan, Yang Yang, Ming Shu, Zeyu Chen, Zeling Zhang, Xiaotong Mei, Weihong Zhong

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Microbial community assembly in the fermentation of cigar tobacco leaves.Frontiers in bioengineering and biotechnology · 2026
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jun Li *College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Fengmei Yi *College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Guoqing ChenCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Fanda PanTechnology Center, China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou, 310009, China.
Yang YangTechnology Center, China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou, 310009, China. Yangy@zjtobacco.com.
Ming ShuTechnology Center, China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou, 310009, China.
Zeyu ChenCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Zeling ZhangCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Xiaotong MeiCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Weihong ZhongCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China. whzhong@zjut.edu.cn.ORCID http://orcid.org/0000-0002-5673-2222
Zhejiang University of Technology · CNChina Tobacco · CN

Funding

National Natural Science Foundation of China 21938012National Natural Science Foundation of China No. 31670115National Natural Science Foundation of China No. 31800118National Natural Science Foundation of China No. 31970104
6 · The paper itself

Abstract

Nicotine-degrading Pseudomonas sp. JY-Q is a preferred strain utilized in reconstituted tobacco process for tobacco waste treatment. However, its efficiency of nicotine metabolism still requires to be improved via genomic technology such as promoter engineering based on genomic information. Concerning upstream module of nicotine metabolic pathway, we found that two homologous genes of nicotine dehydrogenase (nicA2 and nox) coexisted in strain JY-Q. However, the transcriptional amount of nox was 20-fold higher than that of nicA2. Thus, the nicA2 expression required improvement. Combinatorial displacement was accomplished for two predicted endogenous promoters, named as PnicA2 and Pnox for nicA2 and nox, respectively. The mutant with Pnox as the promoters for both nicA2 and nox exhibited the best nicotine metabolic capacity which increased by 66% compared to the wild type. These results suggested that endogenous promoter replacement is also feasible for function improvement of metabolic modules and strain enhancement of biodegradation capacity to meet real environment demand.

Indexed as

Microorganisms, Genetically-ModifiedNicotinePromoter Regions, GeneticPseudomonasBiodegradation, EnvironmentalMetabolic Networks and PathwaysNicotianaSolid WasteNicotineSolid WasteBiodegradationDehydrogenase promoterFunction enhancementNicotinePseudomonas

Identifiers

PMID34061306
OpenAlexW3165770094

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.