Evidence map›Paper›PMID 34060045›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Modified Blue Native Gel Approach for Analysis of Respiratory Supercomplexes.

Sergiy M Nadtochiy, Megan Ngai, Paul S Brookes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sergiy M NadtochiyDepartment of Neuroscience, University of Rochester Medical Center, Rochester, NY, USA.
Megan NgaiDepartment of Anesthesiology, University of Rochester Medical Center, Rochester, NY, USA.
Paul S BrookesDepartment of Anesthesiology, University of Rochester Medical Center, Rochester, NY, USA. paul_brookes@urmc.rochester.edu.

Funding

SIRT1, Nitro-Lipids and CardioprotectionR01HL071158 · NHLBI · UNIVERSITY OF ROCHESTER · PI BROOKES, PAUL S · 2003 to 2025
$7.7M
NHLBI NIH HHS R01 HL071158
6 · The paper itself

Abstract

In mitochondrial oxidative phosphorylation (Ox-Phos), individual electron transport chain complexes are thought to assemble into supramolecular entities termed supercomplexes (SCs). The technique of blue native (BN) gel electrophoresis has emerged as the method of choice for analyzing SCs. However, the process of sample extraction for BN gel analysis is somewhat tedious and introduces the possibility for experimental artifacts. Here we outline a streamlined method that eliminates a centrifugation step and provides a more representative sampling of a population of mitochondria on the final gel. Using this method, we show that SC composition does not appear to change dynamically with altered mitochondrial function.

Indexed as

AnimalsElectron Transport Chain Complex ProteinsHeartMiceMitochondria, HeartMitochondrial ProteinsMultiprotein ComplexesMyocardiumNative Polyacrylamide Gel ElectrophoresisOxidative PhosphorylationElectron Transport Chain Complex ProteinsMitochondrial ProteinsMultiprotein ComplexesBlue-nativeClear-nativeMitochondriaPermeability transition poreRespirationSupercomplexes

Identifiers

PMID34060045
PMCPMC9987619

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.