Evidence map›Paper›PMID 34060035›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes.

Gisela Beutner, George A Porter

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Dysfunctional Electron Transport Chain Assembly in COXPD8.Journal of cardiovascular development and disease · 2025
    Article
  2. Article
  3. Article
  4. Modified Blue Native Gel Approach for Analysis of Respiratory Supercomplexes.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Gisela BeutnerDepartment of Pediatrics-Division Cardiology, University of Rochester, Rochester, NY, USA.
George A PorterDepartments of Pediatrics-Division Cardiology, Pharmacology and Physiology, and Medicine (Aab Cardiovascular Research Institute), University of Rochester, Rochester, NY, USA. George_Porter@urmc.rochester.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Native electrophoresis is a powerful tool to analyze the mitochondrial electron transport chain complexes (Cx) I-V and their assembly into supercomplexes. Valuable information regarding the composition and bioenergetic regulation in physiological and pathological conditions can be obtained. This chapter compares different types of native electrophoresis to analyze mitochondrial supercomplexes.

Indexed as

AnimalsElectron TransportElectron Transport Chain Complex ProteinsElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelHumansImmunoblottingMitochondriaMitochondrial MembranesMitochondrial ProteinsElectron Transport Chain Complex ProteinsMitochondrial ProteinsMitochondrial supercomplexesNative electrophoresis (blue native, colorless native, clear native, hybrid)

Identifiers

PMID34060035

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.