Evidence map›Paper›PMID 34059933›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2021

Comparative analysis of routine parasitological methods for recovery of cysts, molecular detection, and genotyping of Giardia duodenalis.

Renata Coltro Bezagio, Cristiane Maria Colli, Liara Izabela Lopes Romera, Caroline Rodrigues de Almeida, Érika Cristina Ferreira, Mônica Lúcia Gomes

Abstract readComparative Study
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In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Renata Coltro BezagioPostgraduate Program in Health Sciences, State University of Maringá, Maringá, Paraná, Brazil. renata_coltro@hotmail.com.ORCID http://orcid.org/0000-0001-9935-0972
Cristiane Maria ColliDepartment of Basic Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.
Liara Izabela Lopes RomeraPostgraduate Program in Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.
Caroline Rodrigues de AlmeidaPostgraduate Program in Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.
Érika Cristina FerreiraDepartment of Statistics, State University of Maringá, Maringá, Paraná, Brazil.
Mônica Lúcia GomesPostgraduate Program in Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001
6 · The paper itself

Abstract

In order to improve the diagnosis of giardiasis, fecal samples (high/medium/low concentration of cysts) were processed by the parasitological methods used in the routine: Faust, Lutz e Ritchie modified (replacement of formaldehyde by distilled water). The cysts were quantified; the DNA was extracted and amplified by semi-nested PCR (GDH gene). Fifteen clinical samples were analyzed to validate the study by PCR-RFLP. The results showed that the parasite was only detected and genotyped correctly when samples from children with high, medium, and low parasitic load, belonging to genotype AII, were processed by the modified Ritchie method, different from what was observed for the other methods used in laboratory routine (Faust and Lutz). The modified Ritchie method proved to be more suitable, recovering a greater number of cysts from samples, regardless of parasitic load, which reduces the chance of false negative results and has epidemiological repercussions since individuals with low parasite load are usually asymptomatic and the main disseminators of this infection.

Indexed as

Child, PreschoolFecesFemaleGenotypeGenotyping TechniquesGiardia lambliaGiardiasisHumansInfantLife Cycle StagesMaleMolecular Diagnostic TechniquesParasite LoadPolymerase Chain ReactionProtozoan ProteinsProtozoan ProteinsDiagnosisGenotyping techniquesGiardiasisParasite loadPolymerase chain reaction

Identifiers

PMID34059933

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.