ArticleNature methods2021
A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM.
Article in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed.
- A methodology to reduce the localization error in multi-loci microscopy provides new insights into enhancer biology.PLoS computational biology · 2025Article
- ECLiPSE: a versatile classification technique for structural and morphological analysis of 2D and 3D single-molecule localization microscopy data.Nature methods · 2024Article
- Distinct mobility patterns of BRCA2 molecules at DNA damage sites.Nucleic acids research · 2024Article
- Membrane topography and the overestimation of protein clustering in single molecule localisation microscopy - identification and correction.Communications biology · 2024Article
- Temporal analysis of relative distances (TARDIS) is a robust, parameter-free alternative to single-particle tracking.Nature methods · 2024Article
- Disordered C-terminal domain drives spatiotemporal confinement of RNAPII to enhance search for chromatin targets.Nature cell biology · 2024Article
- Disordered C-terminal domain drives spatiotemporal confinement of RNAPII to enhance search for chromatin targets.bioRxiv : the preprint server for biology · 2023Article
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Article
- A framework for evaluating the performance of SMLM cluster analysis algorithms.Nature methods · 2023Article
- Cluster analysis for localisation-based data sets: dos and don'ts when quantifying protein aggregates.Frontiers in bioinformatics · 2023Review
- STORM Super-Resolution Imaging of CBMethods in molecular biology (Clifton, N.J.) · 2023Article
- Recent development of computational cluster analysis methods for single-molecule localization microscopy images.Computational and structural biotechnology journal · 2023Review
- Single-molecule tracking of PprI inFrontiers in microbiology · 2023Article
- Unbiased choice of global clustering parameters for single-molecule localization microscopy.Scientific reports · 2022Article
- High-precision estimation of emitter positions using Bayesian grouping of localizations.Nature communications · 2022Article
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
- Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier.Nature methods · 2022Article
- A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.Nature communications · 2022Article
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13 authors.
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Abstract
Single-molecule localization microscopy (SMLM) relies on the blinking behavior of a fluorophore, which is the stochastic switching between fluorescent and dark states. Blinking creates multiple localizations belonging to the same fluorophore, confounding quantitative analyses and interpretations. Here we present a method, termed distance distribution correction (DDC), to eliminate blinking-caused repeat localizations without any additional calibrations. The approach relies on obtaining the true pairwise distance distribution of different fluorophores naturally from the imaging sequence by using distances between localizations separated by a time much longer than the average fluorescence survival time. We show that, using the true pairwise distribution, we can define and maximize the likelihood, obtaining a set of localizations void of blinking artifacts. DDC results in drastic improvements in obtaining the closest estimate of the true spatial organization and number of fluorescent emitters in a wide range of applications, enabling accurate reconstruction and quantification of SMLM images.
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